The antiparasitic effect of C-Phycocyanin nanoparticles on cryptosporidiosis in immunosuppressed mice.
J Parasit Dis
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, 21321 Egypt.
Published: March 2025
Unlabelled: The present study aimed to newly synthesize C-Phycocyanin nanoparticles through a ball-milling technique. C-Phycocyanin nanoparticles had average diameter of 290.2 nm, zeta potential 48.3 mV and 0.390 PDI. The synthesized nanoparticles were tested as an anti-cryptosporidiosis agent compared to the crude C-Phycocyanin. Cryptosporidiosis induction was done in immunocompetent and immunosuppressed mice. The antiparasitic effect was evaluated through multiplex qualitative PCR, count of oocysts, serum biochemical parameters, oxidative stress and antioxidant markers, cytokine analysis, and histopathological study (qualitative and quantitative). Multiplex qualitative PCR analysis revealed the presence of the tested parasite gene (JVAF) in all the treated groups. The percentage of the highest reduction of the oocysts means counting has been detected in the infected mice treated with nitazoxanide (NTZ) (Ic, IIc) followed by treatment with C-Phycocyanin nano (Ie, IIe), then C-Phycocyanin (Id, IId) (42, 48, 37, 36 15, and 29% respectively). C-Phycocyanin and C-Phycocyanin nanoparticles treated groups dramatically affected the levels of catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA) activity. Moreover, treatment with C-Phycocyanin and C-Phycocyanin nanoparticles significantly reduced cytokines levels (Tumor necrosis factor-alpha (TNF-α), interferon-gamma (INF-γ), and interleukin (IL-13)) in contrast to untreated groups. The histological results in the tissues of mice's ileum which are infected by spp. (positive control) exhibited cellular inflammation in the submucosa and lamina properia, as well as thickening and flattening of the villi. However, the application of nanoparticles allowed the villus to grow further, indicating the nano impact of the cryptosporidiosis treatment.
Supplementary Information: The online version contains supplementary material available at (10.1007/s12639-024-01739-2).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833032 | PMC |
| http://dx.doi.org/10.1007/s12639-024-01739-2 | DOI Listing |
Similar Publications
The antiparasitic effect of C-Phycocyanin nanoparticles on cryptosporidiosis in immunosuppressed mice.
J Parasit Dis
March 2025
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, 21321 Egypt.
Unlabelled: The present study aimed to newly synthesize C-Phycocyanin nanoparticles through a ball-milling technique. C-Phycocyanin nanoparticles had average diameter of 290.2 nm, zeta potential 48.
View Article and Find Full Text PDFFunctional nanoplatform for modulating cellular forces to enhance antitumor immunity via mechanotransduction.
J Control Release
March 2025
Medical 3D Printing Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450000, China. Electronic address:
Immune cells are sensitive to the perception of mechanical stimuli in the tumor microenvironment. Changes in biophysical cues within tumor tissue can alter the force-sensing mechanisms experienced by cells. Mechanical stimuli within the extracellular matrix are transformed into biochemical signals through mechanotransduction.
View Article and Find Full Text PDFSpotlight on the long-term effects of micro/nanoplastics exposure on Spirulina platensis: Algal cells, extracellular polymeric substances, and phycocyanin.
Food Chem
April 2025
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address:
Spirulina platensis (SP) provides humans with proteins and natural pigments. The effects of micro/nanoplastics (MNPs) on SP are of great interest. We focused on the effects of high concentrations (100-300 mg/L) of polystyrene MNPs on SP for 50 days.
View Article and Find Full Text PDFPhycocyanin-based multifunctional microspheres for treatment of infected radiation-induced skin injury.
Biomaterials
June 2025
Department of Plastic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, 322000, China; Zhejiang University-Ordos City Etuoke Banner Joint Research Center, Zhejiang University, Haining, 314400, China. Electronic address:
Radiation therapy is a primary modality for cancer treatment; however, it often leads to various degrees of skin injuries, ranging from mild rashes to severe ulcerations, for which no effective treatments are currently available. In this study, a multifunctional microsphere (PC@CuS-ALG) was synthesized by encapsulating phycocyanin-templated copper sulfide nanoparticles (PC@CuS) within alginate (ALG) using microfluidic technology. Phycocyanin, a natural protein derived from microalgae, shows abilities to scavenge reactive oxygen species, repair radiation-induced damage to skin cells, and ameliorate macrophage-related inflammatory responses.
View Article and Find Full Text PDFPhycocyanin/Hyaluronic Acid Microneedle Patches Loaded with Celastrol Nanoparticles for Synergistic Treatment of Diabetic Nephropathy.
ACS Biomater Sci Eng
January 2025
Henan Provincial Key Laboratory of Kidney Disease and Immunology, Henan Provincial People's Hospital, 7 Weiwu Road, Zhengzhou, Henan Province 450003, P. R. China.
Although multifunctional drug delivery systems have shown significant potential in the treatment of diabetic nephropathy (DN), developing an efficient synergistic drug delivery strategy remains a major challenge. The purpose of this paper is to develop a nanoparticle-loaded microneedle (MN) patch transdermal drug delivery system aimed at achieving blood glucose control and reactive oxygen species (ROS) scavenging for the synergistic treatment of DN. MNs are composed of hyaluronic acid and phycocyanin (PC), both exhibiting excellent biocompatibility and degradation properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.