spp. is a parasitic aquatic mite known to infect freshwater aquatic organisms, especially the marine and freshwater molluscs and few species of sponges. (Acari: Hydrachnida: Unionicolidae) generally infect the freshwater bivalves of the Genus sp. They are usually facultative in nature and can be parasitic at any stage of their life cycle. They cause damage to the gills of the host which harms their normal respiration process. The present work portraits the morphological characters, ultrastructure and DNA barcoding of its mitochondrial gene Cytochrome Oxidase I (COI)(mtCOI), and its taxonomic position was justified by obtaining a phylogenetic tree. The description regarding its morphological characters, ultrastructure and molecular characterization has been presented here. This paper holds the report of a parasitic aquatic mite for the first time from a gastropod molluscan host (Lamarck, 1882), from Diamond Harbour, South 24 Parganas, West Bengal, India.
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http://dx.doi.org/10.1007/s12639-024-01704-z | DOI Listing |
Rice (N Y)
March 2025
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Agricultural College of Yangzhou University, Yangzhou, 225009, China.
Chloroplasts are vital for photosynthesis, and their development necessitates proper expression of chloroplast genes. However, the regulatory mechanisms underlying rice chloroplast gene expression have not been fully elucidated. In this study, we obtained an albino mutant of rice, white seedling and lethal 1 (wsl1), which displays significantly decreased chlorophyll contents and impaired chloroplast ultrastructure.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
March 2025
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Microsporidia are divergent fungal pathogens that employ a unique harpoon-like apparatus called the polar tube (PT) to invade host cells. The long PT is fired out of the microsporidian spore over the course of just a few hundred milliseconds. Once fired, the PT is thought to pierce the plasma membrane of a target cell and act as a conduit for the transfer of the parasite into the host cell, which initiates infection.
View Article and Find Full Text PDFMethods Mol Biol
March 2025
Department of Physiology, School of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.
Alternative experimental models based upon isolation and culture of extra-neural NSC/NPC or adult stem cells that can be induced in vitro to generate these cells and NS rely on development of procedures that can demonstrate the identity of cells as NSC/NPC and their compromise with the neural lineage during culture. Gene expression analyses by RT-PCRQ, immunohistochemical localization of characteristic NSC/NPC antigens, analyses of cell populations by flow cytometry, or ultrastructural analyses of in vitro generated NS are the most frequent and reliable methods to demonstrate the molecular and structural hallmarks of NSC/NPCs and NS. Among all these techniques, those aimed to immunolocalize specific NSC/NPC antigens on a cell basis and in a time-dependent fashion throughout culture require skillful NS processing before setting up the technique.
View Article and Find Full Text PDFCirc Heart Fail
March 2025
Division of Cardiovascular Medicine, School of Medicine, University of California San Diego, La Jolla. (K.F., P.D., J.B., M.C., E.E., Y. Chan, Y.G., V.A.D., V.M., N.D.D., A.D., M.K., K.L.P., F.S., Y. Cho, S.L.).
Background: Muscle proteins of the obscurin protein family play important roles in sarcomere organization and sarcoplasmic reticulum and T-tubule architecture and function. However, their precise molecular functions and redundancies between protein family members as well as their involvement in cardiac diseases remain to be fully understood.
Methods: To investigate the functional roles of Obsc (obscurin) and its close homolog Obsl1 (obscurin-like 1) in the heart, we generated and analyzed knockout mice for , , as well as double knockouts.
Parasit Vectors
March 2025
Nature Research Centre, Akademijos 2, 08412, Vilnius, Lithuania.
Background: The cyst-forming coccidia of the genus Sarcocystis (Sarcocystidae) are widespread protists of mammals, particularly of domestic and wild ruminants. Research on genus Sarcocystis in wild members of the subfamily Caprinae is, however, rather limited. Sarcocystis in the Alpine ibex (Capra ibex) have only been investigated in depth once and then solely by morphological techniques.
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