Objective: To examine the effects of prolonged preferential weight bearing (PWB) and reduced ambulation (RA) on hoof lamellae using a nonpainful in vivo experimental model.
Methods: 12 healthy Standardbred horses were housed in stocks continuously for 92 hours. A platform shoe was placed on 1 forelimb in the PWB group (n = 6) to increase the load on the supporting limb (SL) by approximately 10% bodyweight, whereas the RA group (n = 6) had normal weight bearing. Archived healthy horse (n = 8) samples were used as controls. Histomorphometry and histochemistry (terminal deoxynucleotidyl transferase dUTP nick-end labeling [TUNEL], caspase-3, and targeting protein for Xenopus kinesin-like protein [TPX-2]) results were analyzed using mixed-effects linear regression.
Results: Lesions in multiple limbs from the PWB and RA groups included secondary epidermal lamellae elongation, cell death (mostly TUNEL-positive, caspase-3-negative parabasal keratinocytes), and basal cell proliferation (TPX-2 positive). Lesions were most severe in the PWB group SL, with significant increases (vs control) in mean (95% CI) primary epidermal lamellar (PEL) length (3.7 [95% CI, 3.5 to 3.8] mm vs 3.2 [95% CI, 2.9 to 3.4] mm; P < .001), secondary epidermal lamellae length (281 [95% CI, 235 to 327] µm vs 185 [95% CI, 155 to 215] µm; P < .001), TUNEL count (45 [95% CI, 30 to 60] vs 4 [95% CI, 2 to 5] positive cells/PEL; P < .001), and TPX-2 count (116 [95% CI, 46 to 186] vs 5 [95% CI, 3 to 6] positive cells/PEL; P < .002). Both TUNEL- and TPX-2-positive cell counts were increased in the RA group forelimbs versus control (P < .05).
Conclusions: Restriction of normal ambulation, even in the absence of increased weight bearing, caused lamellar parabasal keratinocyte death and structural derangement of lamellae.
Clinical Relevance: Promoting ambulation, not just limb load relief, may be a critical strategy for preventing SL laminitis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2460/ajvr.24.09.0268 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of a three-week full leg cast immobilization on infant crawling kinetics and spatiotemporal parameters.
PLoS One
March 2025
Wellstar College of Health and Human Services, Kennesaw State University, Kennesaw, Georgia, United States of America.
Despite its importance in the development of mobility in infants, there is a general lack of quantified data on infant crawling, and a specific lack of understanding of crawling kinetics, which includes the relative weightbearing and force generation among the four limbs. Moreover, because of the difficulty of measurement and study design, there is no longitudinal quantification of the impact of a perturbation to typical development, such as limb immobilization. This study measured kinetic and spatiotemporal outcomes in a typically developing infant prior to a fracture that necessitated a long-leg cast that immobilized the left knee and ankle, while crawling with the cast, and again one day after cast removal, and two weeks post.
View Article and Find Full Text PDFOchronotic arthropathy: skeletal manifestations and orthopaedic treatment.
EFORT Open Rev
February 2025
Alkaptonuria is an extremely rare disorder of tyrosine metabolism caused by an autosomal recessive enzymatic deficiency of homogentisic acid (HGA) oxidase, causing its accumulation in collagenous structures, especially in hyaline cartilage. It is characterized by a triad of homogentisic aciduria, bluish-black discoloration of connective tissues (ochronosis) and arthropathy of the spine and large weight-bearing joints. Several clinical manifestations were described including coronary and valvular calcification, aortic stenosis, limited chest expansion, and renal, urethral and prostate calculi as well as ocular and cutaneous pigmentation.
View Article and Find Full Text PDFTwo-stage ankle arthrodesis using the induced membrane technique for pyogenic arthritis: a case report.
J Med Case Rep
March 2025
Department of Orthopaedic Surgery, Teikyo University School of Medicine, 2-11-1, Kaga, Itabashi, Tokyo, 173-8605, Japan.
Background: Ankle arthrodesis is the most frequently performed salvage procedure for pyogenic arthritis. However, its failed fusion rate of approximately 15% has been considered problematic. Herein, we present a case of pyogenic ankle arthritis successfully treated via a two-stage surgical procedure on the basis of the induced membrane technique.
View Article and Find Full Text PDFComputational evaluation of the biomechanical effects of position changes in the femoral neck system on Pauwels type III femoral neck fractures: an in silico study.
Front Bioeng Biotechnol
February 2025
Department of Orthopedics, Luzhou Longmatan District People's Hospital, Luzhou, Sichuan, China.
Introduction: Despite the biomechanical advantages of the Femoral Neck System (FNS), improvements in postoperative complication rates have not been significant. This study evaluated the effects of different FNS positions on the biomechanical stability of Pauwels type III femoral neck fractures (FNFs) using finite element analysis (FEA).
Methods: Pauwels type III FNF models fixed with different FNS positions were constructed using various bolt lengths, bolt positions, and axis-bolt angles.
Background And Aims: Following an ankle sprain, clinical examination often reveals ankle dorsiflexion pain, which has been implicated as a risk factor for recurrent ankle sprains; however, the mechanism of ankle dorsiflexion pain has not been explored. Using magnetic resonance imaging (MRI), we evaluated the relationship between the limited dorsiflexion range of motion due to pain and the position of the talus. We investigated whether an anterior talofibular ligament (ATFL) tear affected ankle dorsiflexion.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!