The possibility is discussed of mapping the E. coli K-12 markers on the basis of mathematical models of recombination previously developed by the authors. The analysis showed that it is advisable to use the symmetrical crossing-over model for mapping the genes close to the selective marker. The stochastic model of recombination gives good results also for genes located in the proximal part of the chromosome.
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PLoS Genet
January 2025
Centre for Immunology and Infection Control, School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD, Australia.
Bacterial polysaccharide synthesis is catalysed on the universal lipid carrier, undecaprenol phosphate (UndP). The cellular UndP pool is shared by different polysaccharide synthesis pathways including peptidoglycan biogenesis. Disruptions in cytosolic polysaccharide synthesis steps are detrimental to bacterial survival due to effects on UndP recycling.
View Article and Find Full Text PDFMicrobiology (Reading)
January 2025
Instituto de Microbiologa, Colegio de Ciencias Biolgicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador.
Antimicrobial resistance (AMR) is a major threat to global public health that continues to grow owing to selective pressure caused by the use and overuse of antimicrobial drugs. Resistance spread by plasmids is of special concern, as they can mediate a wide distribution of AMR genes, including those encoding extended-spectrum -lactamases (ESBLs). The CTX-M family of ESBLs has rapidly spread worldwide, playing a large role in the declining effectiveness of third-generation cephalosporins.
View Article and Find Full Text PDFLife (Basel)
January 2025
Centre sur la Vie dans l'Univers, Université de Genève, 1211 Geneva, Switzerland.
Studying exoplanet atmospheres is essential for assessing their potential to host liquid water and their capacity to support life (their habitability). Each atmosphere uniquely influences the likelihood of surface liquid water, defining the habitable zone (HZ)-the region around a star where liquid water can exist. However, being within the HZ does not guarantee habitability, as life requires more than just liquid water.
View Article and Find Full Text PDFNPJ Antimicrob Resist
April 2024
Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.
Antimicrobial resistance can arise in the natural environment via prolonged exposure to the effluent released by manufacturing facilities. In addition to antibiotics, pharmaceutical plants also produce non-antibiotic pharmaceuticals, both the active ingredients and other components of the formulations. The effect of these on the surrounding microbial communities is less clear.
View Article and Find Full Text PDFbioRxiv
January 2025
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
The activity of DNA adenine methyltransferase (Dam) and DNA cytosine methyltransferase (Dcm) together account for nearly all methylated nucleotides in the K-12 MG1655 genome. Previous studies have shown that perturbation of DNA methylation alters global gene expression, but it is unclear whether the methylation state of Dam or Dcm target sites regulates local transcription. In recent genome-wide experiments, we observed an underrepresentation of Dam sites in transcriptionally silent extended protein occupancy domains (EPODs), prompting us to hypothesize that EPOD formation is caused partially by low Dam site density.
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