Mikrochim Acta
Clinical Laboratory, Clinical Medical Collegeand , The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, 610500, People's Republic of China.
Published: February 2025
A novel electrochemical cytosensor was developed with a three-protein recognition strategy, employing branched Pt Au nanospheres (B-Pt-Au NPs) as tags and MnO-GO-Au nanosheet-modified electrodes for circulating tumor cells (CTC) detection. This system integrates specific magnetic separation and CTC enrichment with sensitive electrochemical detection to identify rare A549 cells in whole blood. MnO-GO-Au nanosheets, known for their high conductivity and biocompatibility, were used to modify a bare electrode, improving the electrochemical interface for biomolecules. The B-Pt-Au NPs could only approach the sensing interface and enhance the current signal through their specific electrocatalytic activity when all three specific proteins, i.e. mucin 1 (MUC1), epithelial cell adhesion molecule (EpCAM), and epidermal growth factor receptor (EGFR), of rare A549 cells in peripheral blood were simultaneously expressed on the cell membrane. The developed cytosensor demonstrated a detection limit as low as 1 cell mL⁻ and, more importantly, could accurately distinguish A549 cells from other cancer cells. Thus, this proposed strategy offers a powerful tool for liquid biopsy analysis of extremely rare CTCs in complex peripheral blood, with potential applications in reliable early diagnosis of non-small cell lung cancer (NSCLC).
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http://dx.doi.org/10.1007/s00604-025-07032-0 | DOI Listing |
Cureus
February 2025
Anatomy, All India Institute of Medical Sciences, Madurai, Madurai, IND.
This review addresses the significant challenges and technological developments in cancer screening and early diagnosis in the context of India's diverse and resource-constrained healthcare landscape. Selected cancers like breast, cervical, oral, lung, and colorectal cancers are focused on, and established screening methods such as clinical breast examination (CBE), mammography, visual inspection with acetic acid (VIA), HPV DNA testing, and oral visual inspection (OVI) are reviewed. These are cost-effective strategies that are proven to reduce mortality.
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Laboratory Medicine Center, Affiliated Chuzhou Hospital of Anhui Medical University, Chuzhou, Anhui, China.
Objective: Acute myeloid leukemia (AML) is a highly heterogeneous hematologic malignancy, with various clinical features influencing its prognosis. The aim of this study to evaluate the impact of platelet count at diagnosis and platelet recovery after induction chemotherapy on the survival outcomes of elderly AML patients.
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Int J Mol Sci
March 2025
Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Gestational diabetes mellitus (GDM) is characterized by an inadequate pancreatic β-cell response to pregnancy-induced insulin resistance, resulting in hyperglycemia. The pathophysiology involves reduced incretin hormone secretion and signaling, specifically decreased glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), impairing insulinotropic effects. Pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), impair insulin receptor substrate-1 (IRS-1) phosphorylation, disrupting insulin-mediated glucose uptake.
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Center of Immunology, Stefan S. Nicolau Institute of Virology, Romanian Academy, 030304 Bucharest, Romania.
Food intake is an essential contributor to both health and disease. Nutrients contribute to a beneficial metabolic equilibrium at the cellular level, preventing or delaying disease onset. Dietary intake contributes to obesity, and obesity supports further cancer and metastasis.
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Division of Medical Oncology, AOU "G. Martino" Hospital, University of Messina, 98125 Messina, Italy.
Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, with metastatic disease posing significant therapeutic challenges. While anti-EGFR therapy has improved outcomes for patients with and wild-type tumors, resistance remains a major hurdle, limiting treatment efficacy. The concept of negative hyperselection has emerged as a refinement of molecular profiling, identifying additional genomic alterations-such as and amplificationsand mutations-that predict resistance to anti-EGFR agents.
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