Absorption spectroscopy is combined with the principle of multiple wavelengths to develop a biomedical sensing mechanism, laid by two Fibre Bragg Gratings. It is essential to incorporate a sample holder in the setup in which the substances can be tested, necessitating its complete investigation without and with the holder, in both directions. The average losses of the fibre junctions are 0.44 and 0.18 dB, respectively, with accuracy of ±0.2 dB which lies within the intensity profile specified by the manufacturer (0.3 dB). Next, the spectral profiles and its respective factors (slope, threshold, mode spacing, intensity levels) of both systems are compared and thoroughly investigated on technical grounds, to examine any anticipated issues for the sensor's operation. Afterwards, we place the holder in the laser setup and check its efficiency by comparing it the intensity profiles of the system without it, under identical parametric values. The average Relative Intensity Noise is found to be consistently low and analogous in both setups, with scientific justifications. Repetition in the forward and reverse directions, and swapping the positions of the lenses, the outcomes show homogenous patterns, which provides conclusive approval with specified parametric regulations in this work.
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http://dx.doi.org/10.1016/j.heliyon.2025.e42294 | DOI Listing |
PLoS One
March 2025
Department of Medicine, University of Wisconsin Hospitals and Clinics, Madison, Wisconsin, United States of America.
Introduction: Infectious diarrheal diseases are one of the leading causes of worldwide morbidity and mortality. The incidence of diarrhea is higher in Low-Middle-Income Countries (LMIC), where more than 90% of deaths from diarrheal diseases occur. Diagnostic tests for infectious diarrhea are not readily available in Low-Middle-Income Countries.
View Article and Find Full Text PDFAnal Chem
March 2025
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China.
In recent years, urgent food safety issues have heightened the demand for rapid detection technologies for foodborne pathogens, especially biosensors featuring simplicity, rapidity, and high sensitivity. Yet despite a booming surge in related published studies, commercializing these biosensors remains a constant challenge and persistent objective for researchers. In this study, a gravity-driven microfluidic chip with tilt-actuated siphon valves was developed, integrating silica magnetic beads based nucleic acid separation and recombinase-aided amplification (RAA) detection of Typhimurium by simple operations along with a portable biosensing device.
View Article and Find Full Text PDFGenome Biol
March 2025
The Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Background: Genome-wide association studies (GWAS) have identified common variants associated with metabolic dysfunction-associated steatotic liver disease (MASLD). However, rare coding variant studies have been limited by phenotyping challenges and small sample sizes. We test associations of rare and ultra-rare coding variants with proton density fat fraction (PDFF) and MASLD case-control status in 736,010 participants of diverse ancestries from the UK Biobank, All of Us, and BioMe and performed a trans-ancestral meta-analysis.
View Article and Find Full Text PDFJ Particip Med
March 2025
Florence Nightingale Faculty of Nursing, Midwifery & Palliative Care, King's College London, London, United Kingdom.
Background: Newborn screening aims to identify babies affected by rare but serious genetic conditions. As technology advances, there is the potential to expand the newborn screening program following evaluation of the likely benefits and drawbacks. To inform these decisions, it is important to consider the family experience of screening and the views of the public.
View Article and Find Full Text PDFAnal Chem
March 2025
Division Biophotonics, Federal Institute for Materials Research and Testing (BAM), Richard-Willstaetter-Strasse 11, Berlin D-12489, Germany.
For a series of 500 μm-thick polyurethane films containing different concentrations of luminescent and scattering YAG:Ce microparticles, we systematically explored and quantified pitfalls of absolute measurements of photoluminescence quantum yields (Φ) for often employed integrating sphere (IS) geometries, where the sample is placed either on a sample holder at the bottom of the IS surface or mounted in the IS center. Thereby, the influence of detection and illumination geometry and sample position was examined using blanks with various scattering properties for measuring the number of photons absorbed by the sample. Our results reveal that (i) setup configurations where the scattering sample is mounted in the IS center and (ii) transparent blanks can introduce systematic errors in absolute Φ measurements.
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