species are essential members of the human gut microbiota, playing crucial roles in host health. Variations in the metabolic functions of different strains can have distinct health effects, making it essential to understand their metabolic characteristics for the development of targeted probiotic formulations. In this study, we cultivated four selected strains from three species: subsp. BB-69, Bbm-19, BX-18, and subsp. B8762. We conducted an untargeted comparative metabolomic analysis to investigate the intracellular metabolic profile and functional activities of these strains. A total of 1,340 metabolites were identified, revealing significant variations in their metabolomic profiles. Notably, subsp. Bbm-19 showed higher activity in amino acid biosynthesis, while subsp. BB-69 exhibited increased activity in secondary bile acid biosynthesis and alpha-linolenic acid metabolism. subsp. B8762 demonstrated enhanced activity in polycyclic aromatic hydrocarbon degradation, vitamin digestion and absorption, and galactose metabolism. BX-18 was more active in tryptophan metabolism and pentose and glucuronate interconversions. Targeted analyses of specific metabolites further revealed strain-specific metabolic pathways. This study systematically elucidates the metabolic profile differences among the four investigated strains at the untargeted metabolomic level, providing valuable insights into their metabolic characteristics and potential applications in probiotic development.
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| http://dx.doi.org/10.3389/fmicb.2025.1522036 | DOI Listing |
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