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Front Cell Infect Microbiol
Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Published: February 2025
Introduction: Vancomycin-resistant (VRE-fm) biofilms pose a significant clinical challenge due to the limited effectiveness of traditional antibiotics. This study investigates the potential of γ-linolenic acid (GLA) as a novel antibiofilm agent.
Methods: Transcriptome analysis was performed on the V27 isolate, comparing cells in mature biofilms treated with and without GLA. The findings were further validated using qRT-PCR on six VRE-fm isolates and two isolates.
Results: Transcriptome analysis revealed a significant downregulation in the expression levels of genes associated with biofilm formation, including , , , , , , , , , and , while a significant upregulation was observed in the expression of . Validation by qRT-PCR in six VRE-fm isolates confirmed the significant changes in the expression levels of all genes except for , with statistical significance. The expression of and genes, which are the homologs of and genes, along with the Fsr-regulated genes and in , were also found to be downregulated by GLA. In addition, KEGG analysis identified specific metabolic pathways that were significantly downregulated by GLA.
Conclusion: GLA effectively targets multiple aspects of biofilm formation in VRE-fm, including the downregulation of key biofilm-related genes, the inhibition of quorum sensing systems, and the modulation of metabolic pathways. GLA emerges as a promising candidate for eradicating biofilms.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826239 | PMC |
http://dx.doi.org/10.3389/fcimb.2025.1525581 | DOI Listing |
Planta
March 2025
School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
The study revealed genetic diversity and moderate differentiation among the R. palmatum complex within the top-geoherb area. RNA-seq-derived SNP datasets hold the potential to trace geographic origins of the core germplasm.
View Article and Find Full Text PDFPlant J
March 2025
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Av. da República, Oeiras, 2780-157, Portugal.
When pollen lands on a receptive stigma, it germinates and extends a tube inside the transmitting tissue of the pistil to deliver the sperm cells for double fertilization. The growth of the pollen tube triggers significant alterations in its gene expression. The extent to which these changes occur in the vegetative cell or extend to the sperm cells transported by the tube is unclear but important to determine since sperm cells are believed to acquire a competency for fertilization during pollen-pistil interactions.
View Article and Find Full Text PDFPlant J
March 2025
Institut de Biologie Physico-Chimique, Laboratoire de Photobiologie et Physiologie des Plastes et des Microalgues, UMR7141 Centre National de la Recherche Scientifique (CNRS), Sorbonne Université, Paris, 75005, France.
Diatoms are prominent microalgae found in all aquatic environments. Over the last 20 years, thanks to the availability of genomic and genetic resources, diatom species such as Phaeodactylum tricornutum and Thalassiosira pseudonana have emerged as valuable experimental model systems for exploring topics ranging from evolution to cell biology, (eco)physiology, and biotechnology. Since the first genome sequencing projects initiated more than 20 years ago, numerous genome-enabled datasets have been generated, based on RNA-Seq and proteomics experiments, epigenomes, and ecotype variant analysis.
View Article and Find Full Text PDFCryo Letters
March 2025
Molecular Biology and Genetics Department, Faculty of Science, Sitki Koçman University, Mugla, Turkiye.
Cryopreservation is a well-known strategy to conserve genetic resources at ultra-low temperature. However, there is still limited knowledge on the cellular processes and molecular adjustments that allow cells to withstand the multiple stresses to which they are exposed during cryopreservation. To evaluate these processes, transcriptomics, the sub-discipline of omics that simultaneously examines mRNA transcripts formed by transcription from the genome, has been recently used.
View Article and Find Full Text PDFJ Gerontol A Biol Sci Med Sci
March 2025
The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 100730, Beijing, China.
The aging of the population is a global concern. In the post-COVID-19 pandemic era, there are no effective methods to identify aging acceleration due to infection. In this study, we conducted whole-transcriptome sequencing on peripheral blood samples from 35 healthy individuals (22-88 years old).
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