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Transcriptome analysis reveals the molecular mechanism of γ-linolenic acid eradicating the biofilm of vancomycin-resistant .
Front Cell Infect Microbiol
Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Published: February 2025
Introduction: Vancomycin-resistant (VRE-fm) biofilms pose a significant clinical challenge due to the limited effectiveness of traditional antibiotics. This study investigates the potential of γ-linolenic acid (GLA) as a novel antibiofilm agent.
Methods: Transcriptome analysis was performed on the V27 isolate, comparing cells in mature biofilms treated with and without GLA. The findings were further validated using qRT-PCR on six VRE-fm isolates and two isolates.
Results: Transcriptome analysis revealed a significant downregulation in the expression levels of genes associated with biofilm formation, including , , , , , , , , , and , while a significant upregulation was observed in the expression of . Validation by qRT-PCR in six VRE-fm isolates confirmed the significant changes in the expression levels of all genes except for , with statistical significance. The expression of and genes, which are the homologs of and genes, along with the Fsr-regulated genes and in , were also found to be downregulated by GLA. In addition, KEGG analysis identified specific metabolic pathways that were significantly downregulated by GLA.
Conclusion: GLA effectively targets multiple aspects of biofilm formation in VRE-fm, including the downregulation of key biofilm-related genes, the inhibition of quorum sensing systems, and the modulation of metabolic pathways. GLA emerges as a promising candidate for eradicating biofilms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11826239 | PMC |
| http://dx.doi.org/10.3389/fcimb.2025.1525581 | DOI Listing |
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