This study aimed to prepare Maillard reaction products (MRPs) from whey protein isolate (WPI) and different reducing sugars (glucose, fructose, maltose, lactose), and utilize the optimal MRPs to fabricate tuna oil (TO) microcapsules for enhancing TO's storage stability. The results showed that the optimal wet heat reaction duration of WPI and reducing sugar was 8 h at 75 °C. Glycosylation improves the functional properties of WPI. WPI-maltose coupling (WPI-M) and Arabic gum were selected as the wall material to prepare TO microcapsules by complex coacervation, and the encapsulation efficiency of microcapsules reached 87.41 %. Compared to WPI, WPI-M microcapsules have a more homogeneous emulsion morphology. The peroxide value of microencapsulated protected TO was 35.78 % lower than that of free TO after accelerated oxidation at 55 °C for 16 days. Microcapsules prepared with MRPs wall materials by complex coacervation offer a promising approach for the preservation of compounds.
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http://dx.doi.org/10.1016/j.foodchem.2025.143269 | DOI Listing |
J Am Chem Soc
March 2025
Institute of Biological Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
In this study, the role of phosphorylation in the liquid-liquid phase separation (LLPS) of tau, the underlying driving forces, and the potential implications of this separation on protein conformation and subsequent protein aggregation were investigated. We compared in vivo-produced phosphorylated tau (p-tau) and nonphosphorylated tau under different coacervation conditions without adding crowding agents. Our findings revealed that spontaneous phase separation occurs exclusively in p-tau, triggered by a temperature shift from 4 °C to room temperature, and is driven by electrostatic and hydrophobic interactions.
View Article and Find Full Text PDFNat Commun
March 2025
Max Planck Institute for Polymer Research, 55128, Mainz, Germany.
Biomolecular condensates formed by proteins and nucleic acids are critical for cellular processes. Macromolecule-based coacervate droplets formed by liquid-liquid phase separation serve as synthetic analogues, but are limited by complex compositions and high molecular weights. Recently, short peptides have emerged as an alternative component of coacervates, but tend to form metastable microdroplets that evolve into rigid nanostructures.
View Article and Find Full Text PDFJ Sci Food Agric
March 2025
School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, People's Republic of China.
Background: The development of acid-soluble soybean protein (ASSP) is a dynamic field with ongoing research aimed at exploring its emulsifying, foaming, and gelling properties. These properties can affect the texture, stability, and sensory attributes of food. Innovations in processing technologies, such as freeze-drying, hydrothermal treatment, and homogenization processing, are being made to enhance the quality of ASSP, which are crucial for its application in food products.
View Article and Find Full Text PDFLangmuir
March 2025
Department of Chemistry and Materials Engineering, Kansai University, 3-3-35, Yamate-cho, Suita, Osaka 564-8680, Japan.
Associative phase separation (complex coacervation) in liquid-liquid phase separation (LLPS) involves the separation of multiple substances into concentrated and dilute phases by electrostatic interactions. Simple phase separation (simple coacervation) occurs when the hydrophilicity and hydrophobicity of a single molecule change dramatically in response to a specific stimulus. Simple coacervation arises from the lower critical solution temperature (LCST)- and upper critical solution temperature (UCST)-type phase separations in aqueous media containing temperature-responsive polymers.
View Article and Find Full Text PDFBiomacromolecules
March 2025
Department of Agriculture, Forestry and Bioresources, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Polymeric microcapsules are useful for drug delivery, microreactors, and cargo transport, but traditional fabrication methods require complex processes and harsh conditions. Coacervates, formed by liquid-liquid phase separation (LLPS), offer a promising alternative for microcapsule fabrication. Recent studies have shown that coacervates can spontaneously form hollow cavities under specific conditions.
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