Correlation-based nanometric localization using lattice SIM and dSTORM in PML nuclear bodies validated by calibration spheres.

We evaluated the Lattice di-SIM 3D structural illumination method with a focus on Promyelocytic Leukemia Nuclear Bodies (PML NBs). Lattice SIM's performance was compared to its predecessor using biological samples and calibration beads. Optimized SIM parameters revealed PML NBs with a distinct ring-like morphology. Furthermore, we compared Lattice-SIM with dSTORM, finding a strong correlation between blinking events and the SIM mask. For 2D nanoscopic correlation, we used the long-lived imaging buffer Eternity, while the modified variant, Eternity-SIM, with a higher refractive index, provided improved 3D correlation for SIM imaging.