The effects of two autochthonous yeast strains (Pichia kudriavzevii and Debaryomyces hansenii) on the physicochemical characteristics, bacterial community structure, and flavour profile of the dry sausages with 40% substitution of NaCl by KCl were evaluated in this study. The results revealed that the inoculation of yeast strains increased the pH and yeast counts of low-sodium sausages. Higher contents of total esters, aldehydes, and ketones were detected in the inoculated sausages (P < 0.05). Based on the results of high-throughput sequencing, the inoculation of P. kudriavzevii decreased the abundance of Lactobacillus, Weissella, and Leuconostoc. However, the inoculation of D. hansenii increased the abundance of Lactobacillus, Weissella and Staphylococcus, which may help to inhibit the growth of pathogenic microorganisms in sausages. Electronic tongue analysis as well as sensory evaluation revealed that D. hansenii reduced bitter, astringent, and metallic tastes (P < 0.05). Overall, D. hansenii can be used as the prospective stater culture to compensate the flavour defects and improve the safety of the dry sausage with NaCl substitutes.
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http://dx.doi.org/10.1016/j.fm.2025.104739 | DOI Listing |
Front Bioeng Biotechnol
February 2025
Department of Molecular Science and Technology and Advanced College of Bio-convergence Engineering, Ajou University, Suwon, Republic of Korea.
The efficient identification of microbial strains capable of producing rare sphingoid bases, such as sphingosine and sphinganine, is critical for advancing microbial fermentation processes and addressing increasing industrial demands. , a non-conventional yeast, naturally overproduces tetraacetyl phytosphingosine (TAPS); however, the production of other valuable sphingoid bases, including sphingosine, sphinganine, and triacetyl sphingosine, remains a key target. In this study, we developed a novel screening method utilizing fluorescein sodium, a selective fluorescent dye that specifically reacts with non-acetylated sphingoid bases-sphinganine, sphingosine, and phytosphingosine-while exhibiting no reactivity with TAPS.
View Article and Find Full Text PDFFront Pharmacol
February 2025
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
Introduction: Bacteriocin P7 was extracted from the cell-free supernatant (CFS) of G7, which is a strain isolated from mangrove plants.
Methods: In this study, the culture conditions of G7 were optimised using an orthogonal test. The (CFS) was subsequently purified by using TA-GF75 gel chromatography, Tiderose Q HP anion chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).
Foods
March 2025
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
The production of strawberry wine is an effective strategy for addressing the significant economic losses caused by strawberry spoilage. In recent years, there has been an increase in consumer demand for quality and flavor diversity in fruit wines. Therefore, it is necessary to develop novel strawberry wine products.
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February 2025
College of Sericulture, Textile and Biomass Science, Southwest University, Chongqing 400715, China.
is a fungus widely used in food fermentation. This study employed microbial technology, combined with microscopic morphological observations and ITS sequence analysis, to isolate, purify, and identify 10 strains of red yeast mold from various products. After the HPLC detection of metabolic products, the M8 strain containing the toxic substance citrinin was excluded.
View Article and Find Full Text PDFFoods
February 2025
College of Life Sciences, Linyi University, Linyi 276005, China.
With a prolonged refrigeration time, dough becomes syruped and the gluten strength is weakened, which negatively affects the texture of the dough. At the same time, differences in the growth and metabolism of different yeasts lead to large differences in the physicochemical properties of the dough and the final quality of the product. Therefore, in this study, suitable additives and non- were selected to minimize the effects of long-term refrigeration on the physicochemical properties and microstructure of fermented doughs.
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