Stimulation of Microcystis aeruginosa by subtoxic concentrations of contaminants: A meta-analysis.

There is growing evidence for hormetic stimulation of Microcystis aeruginosa, a harmful algal bloom (HAB)-forming cyanobacterium, by subtoxic contaminant concentrations. Hence, the first meta-analysis of approximately 4000 dose responses was conducted to evaluate the underlying biological mechanisms, identify variation determinants, and reveal potential implications for algaecides effectiveness. Approximately 30 chemical contaminants caused significant stimulation (95% CI: 72-153%), which persisted in mixtures, regardless the level of mixture complexity. Stimulation by subtoxic antibiotic contamination occurred in the presence or absence of algaecides, highlighting the potential of chemical contamination to lower algaecide efficiency to control the cyanobacterium. The significant stimulation spanned a wide range of contaminant concentrations, from ≤0.0001 to 200 mg L, and the response amplitude varied with concentration and exposure duration, increasing from 16% in less than one day to 27% on average within 2-4 weeks. Various mechanisms regulating the defense system (39-46%) and photosynthetic physiology (10-12%) and determining productivity and yields (19-22%) were enhanced, ultimately resulting in increased population growth (cell density; 21%), growth rate (15%), and survival (39%). Importantly, intracellular and extracellular microcystins (MC-LR, MC-LW, MC-RR, MC-YR) and their release are enriched by 26-29% in tandem with mcyB over-expression (24%) and N (26%) and Ca (17%) enhancement. However, the stimulation degree depended on the specific MC. The findings not only close a significant gap in the scientific understanding of the underlying mechanisms of contaminant-induced stimulation but also provide critical information to improve HAB management and remediation strategies.