In serology, each sample is typically tested individually, one antigen at a time. This is costly and time consuming. Serology techniques should ideally allow recurrent measurements in parallel in small sample volumes and be inexpensive and fast. Here we show that mass cytometry can be used to scale up multiplexed serology testing by leveraging polystyrene beads uniformly loaded with combinations of stable isotopes. We generated 18,480 unique isotopically barcoded beads to simultaneously detect, in a single tube with 924 serum samples, the levels of immunoglobulins G and M against 19 proteins from SARS-CoV-2 (a total of 36,960 tests in 400 nl of sample volume and 30 μl of reaction volume). As a rapid, high-throughput and cost-effective technique, serology by mass cytometry may contribute to the effective management of public health emergencies originating from infectious diseases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/s41551-025-01349-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Single Multiplex CytoBas Assay Incorporating Eight Major Components for Accurate Detection of Allergen Sensitization in Asthma and Allergic Rhinitis.
Allergy
March 2025
Department of Immunology, School of Translational Medicine, Monash University, Melbourne, Victoria, Australia.
Background: Allergic rhinitis and asthma can be triggered by a variety of aeroallergens, including house dust mites (HDM), tree and grass pollen, and household pets. Identification of the relevant allergen is critical for lifestyle changes and treatments, including allergen immunotherapy. We here assessed the diagnostic performance and clinical utility of a single flow cytometry staining of basophils with major aeroallergen components (AeroDiff CytoBas).
View Article and Find Full Text PDFPost-treatment monitoring of surgically treated oropharyngeal squamous cell carcinoma patients using human papillomavirus cell-free DNA.
Oral Oncol
March 2025
Division of Infections and Cancer Epidemiology, Immunology, Infection & Cancer Research Program, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Introduction: The incidence rate of human papillomavirus (HPV)-driven oropharyngeal squamous cell carcinoma (OPSCC) is increasing. Despite good prognosis, recurrence can decrease health-related quality of life and increase mortality, so post-treatment monitoring is important for patient outcomes. One potential biomarker for post-treatment monitoring is HPV cell-free DNA (cfDNA) from blood plasma.
View Article and Find Full Text PDFHigh-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.
Nat Biomed Eng
February 2025
Department of Pathology, Stanford University, Stanford, CA, USA.
In serology, each sample is typically tested individually, one antigen at a time. This is costly and time consuming. Serology techniques should ideally allow recurrent measurements in parallel in small sample volumes and be inexpensive and fast.
View Article and Find Full Text PDFA Decrease of Antibodies Against SARS-CoV-2 Antigens Does Not Reflect a Decrease of Neutralization Rate: A Prospective Study to Evaluate Kinetic and Dynamic Humoral Immune Response After Vaccination During Pregnancy.
Am J Reprod Immunol
February 2025
Laboratory of Molecular Virology, Carlos Chagas Institute/Fiocruz, Curitiba, Paraná, Brazil.
Problem: Despite being at increased risk for severe COVID-19, pregnant women were initially excluded from vaccine clinical trials, which limited data regarding vaccine effectiveness and protection in this group. Aiming to better understand the immune response to vaccination during pregnancy, we compared the kinetics and titers of neutralizing and IgG antibodies generated against SARS-CoV-2 during vaccination with BNT162b2 (Pfizer-BioNTech) or CoronaVac (Sinovac Biotech) in a cohort of pregnant women.
Methods Of Study: We evaluated participants before vaccination and 30 days after each vaccine dose, using a multiplex bead assay to measure IgG antibodies against SARS-CoV-2 antigens (total spike, spike-1, spike-2, receptor binding domain, and nucleocapsid) and a live virus fluorescence reduction neutralization assay (FRNA) to quantify neutralizing antibodies.
Assessing the Feasibility of Using a Multiplex Serological Assay to Conduct Serosurveillance for Malaria Exposure in Deployed Military Personnel.
Trop Med Infect Dis
January 2025
Biologics Research & Development Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Reproducibly assessing malaria exposure is critical for force health protection for military service members deployed to malaria-endemic regions as well as for civilians making public health decisions and evaluating malaria eradication efforts. However, malaria disease surveillance is challenged by under-reporting, natural immunity, and chemoprophylaxis, which can mask malaria exposure and lead to an underestimation of malaria prevalence. In this study, we determined the feasibility of using a serosurveillance-based approach to measure Anopheles vector exposure, Plasmodium sporozoite exposure, and blood-stage parasitemia using a multiplex serological panel.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!