Background And Purpose: The dorsal nucleus (Clarke's nucleus, D), lumbar precerebellar nucleus (LPrCb), and sacral precerebellar nucleus (Stilling's sacral nucleus, SPrCb) are precerebellar nuclei of the spinal cord. This study investigates the cytoarchitecture and neurochemical organization of the D, LPrCb, and SPrCb nuclei in the human spinal cord.
Material And Methods: Using Nissl staining and immunohistochemistry for markers including calbindin (Cb), calretinin (Cr), parvalbumin (Pv), choline acetyltransferase (ChAT), glutamic acid decarboxylase (GAD 65/67), and vesicular glutamate transporter 1 (VGLUT1), we analyzed sections from T1-T12, L1-L5, and S1-Co1 segments of a human spinal cord.
Results: Our findings reveal a diverse range of neuron sizes and morphologies within these nuclei, with multipolar neurons being predominant. The immunohistochemical analysis showed distinct neurochemical characteristics, with varying densities of the markers across the D, LPrCb, and SPrCb.
Conclusion: This study provides the first detailed characterization of these nuclei in the human spinal cord, highlighting their intricate organization and suggesting potential functional similarities. The comprehensive understanding of the neurochemical profiles of these nuclei lays the groundwork for future research into their roles in motor coordination and their involvement in neurodegenerative diseases. Our findings underscore the importance of further investigation into the pathological changes occurring within the precerebellar nuclei to advance treatment and prevention strategies for related neurological disorders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aanat.2025.152390 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Attenuates Experimental Periodontitis in Mice Partly by Delivering miRNAs.
Int J Nanomedicine
March 2025
Department of Periodontics and Mucosa, The second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
Introduction: Periodontitis is the most common non-communicable disease in humans. The main challenge in the treatment of periodontitis is to effectively control periodontal inflammation and promote tissue repair. Human umbilical cord mesenchymal stem cells-derived exosomes (hucMSCs-exo) have been reported to modulate inflammatory responses and promote tissue repairment mainly through miRNAs in several diseases.
View Article and Find Full Text PDFMotor Control Exercises and Their Design for Short-Term Pain Modulation in Patients with Pelvic Girdle Pain: A Narrative Review.
Healthcare (Basel)
March 2025
Department of Human Neurosciences, Sapienza University of Rome, 00185 Rome, Italy.
Pelvic girdle pain (PGP) is described in the literature as a subgroup of low back pain (LBP), characterized by pain localized between the posterior iliac crest and the gluteal fold, particularly near the sacroiliac joints. This condition can manifest in different forms non-specific PGP, occurring during pregnancy or postpartum (pregnancy-related PGP), which represents the most prevalent form and non-pregnancy-related PGP, resulting from mechanical alterations caused by trauma or microtrauma. Specific PGP, associated with identifiable causes such as fractures, infections, or arthritis.
View Article and Find Full Text PDFMolecular Anatomy of Synaptic and Extrasynaptic Neurotransmission Between Nociceptive Primary Afferents and Spinal Dorsal Horn Neurons.
Int J Mol Sci
March 2025
Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary.
Sensory signals generated by peripheral nociceptors are transmitted by peptidergic and nonpeptidergic nociceptive primary afferents to the superficial spinal dorsal horn, where their central axon terminals establish synaptic contacts with secondary sensory spinal neurons. In the case of suprathreshold activation, the axon terminals release glutamate into the synaptic cleft and stimulate postsynaptic spinal neurons by activating glutamate receptors located on the postsynaptic membrane. When overexcitation is evoked by peripheral inflammation, neuropathy or pruritogens, peptidergic nociceptive axon terminals may corelease various neuropeptides, neurotrophins and endomorphin, together with glutamate.
View Article and Find Full Text PDFExpansion of Splice-Switching Therapy with Antisense Oligonucleotides.
Int J Mol Sci
March 2025
Department of Pediatrics, Hyogo Medical University, 1-1 Mukogawacho, Nishinomiya 663-8501, Japan.
Since 2016, splice-switching therapy, in which splicing is controlled by antisense oligonucleotides, has been applied in clinical practice for spinal muscular atrophy and Duchenne muscular dystrophy. In the former disease, this therapy induces exon inclusion, while, in the latter, it induces exon skipping, leading expression of functional proteins. Basic and clinical studies of splice-switching therapy for many monogenic diseases have now been conducted.
View Article and Find Full Text PDFExploring the Prevalence of Duplication and Deletion in Russia and Its Impact on Carrier Screening.
Int J Mol Sci
February 2025
Research Centre for Medical Genetics, 1 Moskvorechye St., Moscow 115522, Russia.
5q spinal muscular atrophy (5q SMA) is one of the most prevalent autosomal recessive disorders worldwide. In 5q SMA, cases of silent carriers have been reported, including duplications, intragenic subtle variants, de novo variants, and mosaicism. This study included DNA samples from 3412 unexamined unrelated individuals with no known family history of 5q SMA.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!