In this study, we sampled populations of Scorpion Mud Turtles, Kinosternon scorpioides, in rivers and flooded plains of the eastern Brazilian Amazon in order to obtain information on its reproductive biology as well as the geospatial description of its habitat. The research was conducted in the basins of the Pericumã and Aurá Rivers, located in the environmental protection area of the Baixada Maranhense. Seven collections were made over a period of 24 months; 206 animals were captured (101 males and 105 females), with 18 recaptures (8.6%) and a displacement record of 7.41 km. In the Aurá River basin, we recorded six nests of K. scorpioides buried in association with a plant of the genus Andropogon sp. Turtles were observed in a variety of environments in a distribution completely dependent on seasonal changes in its habitat, fluctuating between aquatic and terrestrial microhabitats along with seasonal rains and dry periods and food availability. This study adds data on the ecology and knowledge of the dynamics and habitats of K. scorpioides in relation to conservation strategies of these and other turtles that use these flooded plains as habitat and survive despite many anthropogenic disturbances in the region.
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http://dx.doi.org/10.1590/1519-6984.285306 | DOI Listing |
Genome Res
March 2025
Department of Microbiology and Molecular Genetics, University of California, Davis, California 95616, USA;
As transposable elements (TEs) coevolved with the host genome, the host genome exploited TEs as functional regulatory elements of gene expression. Here we show that a subset of KRAB domain-containing zinc-finger proteins (KZFPs), which are highly expressed in mitotically dividing spermatogonia, repress the enhancer function of endogenous retroviruses (ERVs) and that the release from KZFP-mediated repression allows activation of ERV enhancers upon entry into meiosis. This regulatory feature is observed for independently evolved KZFPs and ERVs in mice and humans, suggesting evolutionary conservation in mammals.
View Article and Find Full Text PDFGen Comp Endocrinol
March 2025
Department of Biological Sciences, Texas Tech University, Box 43131, Lubbock, TX 79409, USA. Electronic address:
Weddell seal (Leptonychotes weddellii) females lose substantial body mass across an intensive, nutritionally restricted lactation period and then must rapidly recover mass during the short Antarctic summer. In this study, we examined endocrine dynamics associated with mass loss across lactation and subsequent realimentation in Weddell seals, comparing patterns between seals that recently gave birth and demographically similar non-reproductive females (skip females) in McMurdo Sound, Antarctica. Postpartum seals near weaning (∼35 days postpartum, n = 64) and skip females (n = 32) were handled during early austral summer (November/December) and rehandled in late summer (January/February).
View Article and Find Full Text PDFDev Biol
March 2025
Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Byblos, Lebanon. Electronic address:
In vitro fertilization, cryopreservation and pre-implantation genetic testing are transformative reproductive technologies offering hope for individuals facing fertility challenges. Scientifically, understanding the science of developmental biology is essential for comprehending the mechanisms and implications of these technologies. In embryological sciences, biological perspectives identify life as a series of developmental stages ranging from conception to viability, each potentially representing a different 'beginning' of life.
View Article and Find Full Text PDFMol Metab
March 2025
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA; Howard Hughes Medical Institute, Dallas, TX, 75390, USA; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA. Electronic address:
The bi-functional enzyme FicD catalyzes AMPylation and deAMPylation of the endoplasmic reticulum chaperone BiP to modulate ER homeostasis and the unfolded protein response (UPR). Human hFicD with an arginine-to-serine mutation disrupts FicD deAMPylation activity resulting in severe neonatal diabetes. We generated the mFicD mutation in mice to create a pre-clinical murine model for neonatal diabetes.
View Article and Find Full Text PDFSTAR Protoc
March 2025
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USA. Electronic address:
When investigating cellular responses to viral infections, there is a need to quantitatively distinguish between dying cells, infected cells, and cells that are both infected and dying. Here, we present a protocol to simultaneously detect porcine reproductive and respiratory syndrome virus (PRRSV) infection and cell death using a flow cytometry double-staining approach. We describe steps for cell seeding, infection, and collection; staining dead cells; sample fixation; and cell permeabilization.
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