The field of reconstructive and regenerative therapy has shown an increased amount of interest in three-dimensional (3D) bioprinting techniques in recent years. This technique applies 3D printing methods to tissue engineering, utilizing additive manufacturing techniques and bio-inks containing biomaterials and living cells. 3D bioprinting has the potential to create the lost tissue precisely. It provides control over the bio-ink component and printing structure, enabling the creation of spatially diverse constructs for the treatment, regeneration, and restoration of various maxillofacial abnormalities, and appears to be a promising alternative. As a result, this review aims to discuss the advancements in 3D bioprinting, including its multiple applications in regenerative and reconstructive dentistry, as well as future perspectives, such as the evolution of 4D bioprinting.
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http://dx.doi.org/10.4103/jpbs.jpbs_678_24 | DOI Listing |
Biotechnol Prog
March 2025
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC, USA.
The organ transplantation field requires new approaches for replacing and regenerating tissues due to the lack of adequate transplant methods. Three-dimensional (3D) extrusion-based bioprinting is a rapid prototyping approach that can engineer 3D scaffolds for tissue regeneration applications. In this process, 3D printed cell-based constructs, consisting of biomaterials, growth factors, and cells, are formed by the extrusion of bioinks from nozzles.
View Article and Find Full Text PDFSovrem Tekhnologii Med
March 2025
DSc, Professor, Institute of Regenerative Medicine; Chief Scientific Officer of the Scientific and Technological Park of Biomedicine; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8/2 Trubetskaya St., Moscow, 119991, Russia.
Most tissues and organs are based on cells of the epithelial and mesenchymal phenotypes. Epithelial cells build protective barriers, have a key role in absorption and secretion, and participate in metabolism. Characterized by high plasticity and ability to migrate, mesenchymal cells ensure structural support, promote tissue restoration and are important for matrix remodeling.
View Article and Find Full Text PDFMater Today Bio
April 2025
Shanghai Clinical Research and Trial Center, Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, 201210, China.
The mechanical properties and physical confinement of the extracellular matrix (ECM) are crucial roles in regulating tumor growth and progression. Extensive efforts have been dedicated to replicating the physical characteristics of tumor tissue by developing two-dimensional (2D) and three-dimensional (3D) models. However, it remains a significant challenge to modulate the local microenvironment around the specific cells according to the culture progress.
View Article and Find Full Text PDFBiomater Sci
March 2025
School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China.
Vascularization is a crucial aspect of biofabrication, as the development of vascular networks is essential for tissue survival and the optimization of cellular functions. Spheroids have emerged as versatile units for vascularization, demonstrating significant potential in angiogenesis and prevascularization for tissue engineering and regenerative medicine. However, a major challenge in creating customized vascularized spheroids is the construction of a biomimetic extracellular matrix (ECM) microenvironment.
View Article and Find Full Text PDFDermatol Surg
March 2025
All authors are affiliated with the Clinical Department of Surgical Oncology, Medical University of Silesia, Katowice, Poland.
Background: The high mortality rate and long treatment time for burns require the development of new effective therapies.
Objective: The study aims to systematically review current research on 3D printing technologies for developing advanced burn dressings, identifying existing challenges and exploring the potential for clinical integration.
Materials And Methods: This systematic review examines 45 studies from 2018 to 2023 on 3D printing technologies for burn dressings to identify challenges and clinical perspectives.
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