Endophytic microbial communities and functional shifts in Hemarthria compressa grass in response to Silicon and Selenium amendment.

Background: Hemarthria compressa, a widely cultivated forage grass, is critical for supporting livestock production and maintaining the ecological balance in grassland ecosystems. Enhancing its stress resistance and productivity is crucial for sustainable grassland utilization and development. Silicon (Si) and Selenium (Se) are recognized as beneficial nutrients that promote plant growth and stress tolerance, and modulate of plant-microorganism interactions. However, the intricate linkages between the endophytes shifts and host grass growth induced by Si/Se amendments are poorly understood. In this study, a pot experiment was conducted to examine the effects of foliar-applied Si/Se on the growth and nutritional quality of H. compressa grass, as well as the composition, diversity and potential functions of endophytic bacteria in leaves.

Results: Both Si and Se treatments significantly improved grass biomass by approximately 17%. Nutritional quality was also improved, with Si application increased plant Si and neutral detergent fiber contents by 25.6% and 5.8%, while Se significantly enhanced the grass Se content from 0.055 mg kg to 0.636 mg kg. Furthermore, Si/Se amendments altered the structure of the leaf endophytic bacterial community, resulting in an increased alpha diversity and a more modularized co-occurrence network. Moreover, both Si and Se treatments enriched plant growth-promoting bacterial genera such as Brevundimonas and Truepera. Metabolic function analysis revealed that Si application promoted chlorophyllide biosynthesis by 152%, several carbon metabolism pathways by 35-152%, and redox-related pathways by 57-93%, while the starch biosynthesis pathway was downregulated by 79% of the endophytic bacterial community. In contrast, Se application mainly enhanced starch degradation, CMP-legionamine biosynthesis by 71% and TCA cycle-related pathways by 23-58%, while reducing L-threonine metabolism by 98%. These specific functional changes in the endophytic bacteria induced by Si/Se amendments were closely linked with the observed growth promotion and stress resistance of the host H. compressa grass.

Conclusions: Si and Se amendments not only enhanced the growth and nutritional quality of H. compressa grass, but also altered the community structure and functional traits of endophytic bacteria in grass. The enrichment of beneficial endophytes and the modification of community metabolic functions within the endophytic community may play important synergistic effects on improving grass growth.