Herbaspirillum species are typically isolated from plants and are known for their role in crop nitrogen fixation. Recently, they have been found to colonize humans and cause infections. This study aimed to identify and characterize two Herbaspirillum huttiense strains (CLJ01 and CLJ02) isolated from the blood of a uremic patient, with a focus on evaluating their pathogenicity and antibiotic efficacy. The strains were identified using the VITEK2 system, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry , and 16S rRNA gene sequencing, confirming their identity as H. huttiense. High-throughput sequencing further revealed the presence of the resistance gene arnA, which confers resistance to polymyxin. For the first time, the pathogenicity of H. huttiense was assessed using a Galleria mellonella infection model. The results demonstrated a concentration-dependent virulence, with CLJ01 exhibiting slightly higher pathogenicity. Additionally, meropenem showed significant antimicrobial efficacy in the G. mellonella infection model, particularly under conditions of high bacterial load, indicating strong therapeutic potential. In conclusion, this study provides experimental evidence supporting the correct diagnosis and treatment of H. huttiense infections. Furthermore, the findings underscore the importance of accurately identifying rare pathogens in clinical settings for effective treatment.
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The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.
Lett Appl Microbiol
February 2025
The Second Affiliated Hospital of Henan University of Chinese Medicine, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China.
Herbaspirillum species are typically isolated from plants and are known for their role in crop nitrogen fixation. Recently, they have been found to colonize humans and cause infections. This study aimed to identify and characterize two Herbaspirillum huttiense strains (CLJ01 and CLJ02) isolated from the blood of a uremic patient, with a focus on evaluating their pathogenicity and antibiotic efficacy.
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Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime, Japan.
In the non-phosphorylative L-rhamnose and L-fucose pathways in bacteria, the C4-OH groups of the L-2-keto-3-deoxyrhamnonate (L-KDR) and L-2-keto-3-deoxyfuconate (L-KDF) intermediates are oxidized by different NAD+-dependent dehydrogenases, which belong to the same superfamily; L-KDRDH and L-KDFDH, respectively. To further elucidate their opposite stereospecificities, we herein investigated the crystal structures of L-KDRDH (from Herbaspirillum huttiense) in ligand-free and NAD+-bound forms. The interactions between the side chains of Asp39 and Gln18, and the 2'- and/or 3'-hydroxyl group(s) of NAD+ were consistent with strict specificity for NAD+.
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Plant Pathology Department, University of Florida, Gainesville, FL, USA.
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Article Synopsis
- Herbaspirillum spp. is a bacterium typically found in environments like soil and water, and is rarely linked to infections, especially in healthy individuals.
- A 57-year-old male with no neutropenia experienced symptoms like fever and malaise after undergoing home dialysis, leading to the discovery of Herbaspirillum huttiense in his blood cultures.
- After treating him with various antibiotics and removing his central line, he recovered without complications, raising the significance of this rare infection in a non-neutropenic dialysis patient.
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