Vulvar lichen sclerosus (VLS) is a chronic, inflammatory, and progressive skin disease mainly involved in the anogenital area. Platelet-rich fibrin (PRF) is a fibrin adhesive-concentrated platelet-rich plasma (PRP) used for tissue repair and angiogenesis. In this study, we explored the effects of PRF on VLS patients, further to utilize the established VLS animal model, to confirm the therapeutic effect of PRF and regulation on the TGF-β/SMAD3 pathway. Among the 46 VLS patients included in the analysis, injectable RPF (i-PRF) treatment improved the symptoms of VLS. The immunohistochemical analysis showed that i-PRF decreased the local blurring of the dermal-epidermal boundary and increased the number of basal keratinocytes. I-PRF increased positive PGD9.5, CD34, and Melan A cell numbers, and decreases positive IL-17 and INF-γ cell numbers in VLS tissues. In the VLS rat model, i-PRF reduced inflammatory factors IL-17 and INF-γ via inhibition of NF-κB and increased CD31 and VEGF expression in external genital tissue. The i-PRF decreases fibronectin and collagen-I by inhibiting TGF-β/SMAD3 in VLS, which is the main factor that triggers inflammation and fibrosis of the external genital skin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00403-025-03811-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bioactivity of dressings based on platelet-rich plasma and Platelet-rich fibrin for tissue regeneration in animal model.
World J Biol Chem
March 2025
Programa Multicêntrico de Pós-graduação em Bioquímica e Biologia Molecular - Setor Palotina, Universidade Federal do Paraná, Palotina 85950-000, Paraná, Brazil.
Background: Skin wounds are common injuries that affect quality of life and incur high costs. A considerable portion of healthcare resources in Western countries is allocated to wound treatment, mainly using mechanical, biological, or artificial dressings. Biological and artificial dressings, such as hydrogels, are preferred for their biocompatibility.
View Article and Find Full Text PDFObservation on efficacy of autologous platelet rich fibrin combined with ultra-pulsed carbon dioxide laser in the treatment of cancellous bone exposed wounds.
Medicine (Baltimore)
March 2025
Department of Burn and Plastic Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China.
This study is to evaluate the effectiveness of autologous platelet-rich fibrin (PRF) combined with ultra-pulsed carbon dioxide laser in treating cancellous bone-exposed wounds. A total of 65 patients with cancellous bone-exposed wounds were retrospectively analyzed selected. There were 35 cases in ultra-pulsed carbon dioxide laser + PRF group (Laser group, group L), and 30 cases in routine surgical debridement + PRF group (Routine group, group R).
View Article and Find Full Text PDFDoes Combining Arthrocentesis With Injectable Platelet-Rich Fibrin Outperform Arthrocentesis or Injectable Platelet-Rich Fibrin Alone in Alleviating Pain and Improving Function in Temporomandibular Joint Dysfunction?
J Oral Maxillofac Surg
February 2025
Professor and Head, Department of Dentistry, Division of Craniomaxillofacial Surgery, All India Institute of Medical Sciences (AIIMS), Rishikesh, India.
Background: Arthrocentesis (AC), with/without intra-articular agents like hyaluronic acid, steroids, platelet-rich plasma, and platelet-rich fibrin, is used with varying effectiveness to manage temporomandibular joint (TMJ) intra-articular pain and dysfunction (IPD).
Purpose: The purpose of this study was to measure changes in pain, range of motion, and quality of life (QOL) in subjects with IPD who underwent AC/injectable platelet-rich fibrin (i-PRF)/AC + i-PRF injection. Study design, setting and sample: A single-centre randomized clinical trial was conducted at All India Institute of Medical Sciences, Rishikesh, on patients with unilateral IPD (Wilkes II, III, and IV) confirmed by magnetic resonance imaging.
This study compared the efficacy of apicoectomy and apical curettage with and without leukocyte- and platelet-rich fibrin (L-PRF) in treating large periapical lesions. Sixty-four adults (30 male, 34 female) with a previously endodontically treated tooth and a large periapical lesion were randomized into four groups (n = 16): retrograde obturation (RG), orthograde obturation (OG), RG + L-PRF, and OG + L-PRF. All participants underwent root canal retreatment in two sessions.
View Article and Find Full Text PDFExosome-Mediated Alveolar Ridge Augmentation: A First Human Case Report with Histology.
Int J Periodontics Restorative Dent
March 2025
Exosomes, the smallest subset of extracellular vesicles, play a crucial role in cell signaling and communication throughout the body. Their regenerative potential has sparked tremendous interest, with over 5,000 articles on exosomes being published yearly, primarily focused on invitro and pre-clinical studies. However, to date, no study has investigated their use in humans for dental applications.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!