Background: The cause of oligoasthenoteratozoospermia (OAT) is unclear. In this original study, we examined OAT patients' sperm using RNA-sequencing and studied the impact of curcumin on gene expression during sperm cryopreservation and freeze-thawing.
Methods: RNA-seq was performed using the Galaxy Europe server in IKHC (Imam Khomeini Hospital Complex), Tehran, Iran in 2023. Sperm samples were collected from 30 OAT patients and 30 healthy volunteers. Sperm parameters were analyzed, and samples were frozen with 20 μM curcumin at -196 °C for one week. Thawed samples were assessed for sperm parameters, and the expression levels of , , , , , , , , and genes were measured using RT-PCR.
Result: RNA-seq analysis showed increased expression of , , and genes, while , , and genes were down-regulated in OAT patients' sperm. Curcumin (20 μM) effectively preserves sperm parameters and motility during cryopreservation in healthy subjects and in OAT patients in particular. Cryopreserved sperm from both OAT patients and healthy individuals exhibited reduced expression of pro-apoptotic genes, increased expression of anti-apoptotic genes, and elevated levels of and genes.
Conclusion: Altered expressions of , , , , , and genes likely contribute to OAT development. Additionally, curcumin protects sperm parameters during cryopreservation for both healthy individuals and OAT patients.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787840 | PMC |
| http://dx.doi.org/10.18502/ijph.v54i1.17593 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Spatial Epidemiologic Analysis of Opioid Use Disorder Treatment in New York State.
Med Care
March 2025
New York State Office of Addiction Services and Supports, New York, NY.
Background: Opioid agonist treatments (OAT; methadone and buprenorphine) for opioid use disorder (OUD) reduce overdose death by more than 50%. Low population-level rates of OAT are missed opportunities to reduce OUD-related mortality.
Objective: We examined county-level OAT utilization patterns to guide state-level and county-level initiatives to improve equitable access and utilization in New York State (NYS).
Dispensing issues and disciplinary measures during opioid agonist treatment: A viewpoint.
J Opioid Manag
March 2025
National Drug Dependence Treatment Center, All India Institute of Medical Sciences, New Delhi.
Opioid Use Disorder (OUD) poses a significant public health challenge globally, with an estimated 23 million opioid users in India. Opioid Agonist Therapy (OAT) stands as the cornerstone of treatment, offering potential reductions in morbidity, societal burdens, and improvements in patient quality of life. However, OAT coverage remains fairly low in India, with clinical guidelines lacking comprehensive information on implementation pragmatics.
View Article and Find Full Text PDFInitial motility and vitality predict the semen quality after long-term cryostorage, even in patients with restricted ejaculate parameters.
Andrology
March 2025
Department of Clinical and Surgical Andrology, Centre of Reproductive Medicine and Andrology, University Hospital of Münster, Münster, Germany.
Background: Cryopreservation of human semen is the cornerstone for preserving male fertility before gonadotoxic therapy or in cases of high variability in semen parameters. This is particular crucial in cases of severe oligoasthenoteratozoospermia (OAT), where diminished sperm counts may compromise planned intracytoplasmic sperm injection (ICSI) procedures. Previous investigations in donor programs have shown long-term storage effects, such as decreased motility in cryopreserved semen samples.
View Article and Find Full Text PDFOSA pathophysiology: a contemporary update.
Aust Dent J
March 2025
Division of Pulmonary, Critical Care, and Sleep Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Defined as an elevated frequency of obstructive respiratory events in sleep, obstructive sleep apnoea (OSA) is driven by a combination of four pathophysiologic mechanisms: elevated upper airway collapsibility, unstable ventilatory control, impaired upper airway dilator muscle responsiveness and decreased arousal threshold. Established therapies such as continuous positive airway pressure (CPAP) and oral appliance therapy (OAT) work chiefly through targeting elevated collapsibility, which affects the majority of OSA patients. However, many patients respond poorly or do not tolerate these 'anatomic' therapies.
View Article and Find Full Text PDFWrist joint tuberculosis masquerading as traumatic arthritis sequalae.
BMC Musculoskelet Disord
March 2025
First College of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, 250355, China.
Background: Wrist joint tuberculosis(WJ-TB)is a rare manifestation of osteoarticular tuberculosis(OAT).Delayed diagnosis is common and often leads to postponed treatment, resulting in complications such as joint stiffness and persistent pain in the affected limb.
Case Presentation: A 50-year-old man presented with symptoms in the wrist joint suggestive of WJ-TB.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!