Endocrine disruptors represent a diverse array of chemicals known to interfere with the endocrine systems of both human and environmental organisms, adversely affecting reproduction, development, and behavior, thus raising significant health and ecological concerns. Traditional regulatory tests for endocrine activity typically involve juvenile or adult fish, which is both time-consuming and resource-intensive and necessitates substantial animal use. This study adopts a transcriptomic approach to identify toxicogenomic signatures associated with the disruption of estrogen signaling in zebrafish (Danio rerio) embryos. Utilizing a modified zebrafish embryo toxicity test based on Organisation for Economic Co-operation and Development test guideline 236, the embryos were exposed to two sublethal concentrations of estradiol, bisphenol A, and fulvestrant. Despite no significant effects on survival or hatching rate observed in treated groups compared with the controls, our study effectively pinpointed several genes, including vtg1, cyp19a1b, fam20cl, sult1st2, pck1, agxtb, hsd17b12a, ptgs2a, and ccn1, as linked to a disruption of estrogen signaling. These genes emerge as promising biomarker candidates for identifying and distinguishing estrogen-related modes of action. Additionally, this approach not only supports the detection of potential endocrine disruptors but also opens up possibilities for prioritizing substances for higher tier endocrine testing, which could substantially reduce animal testing in the future.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/etojnl/vgae059 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identifying prognostic biomarkers and immune interactions in ovarian cancer associated with perfluorooctanoic acid exposure: Insights from comparative toxicogenomics and molecular docking studies.
Ecotoxicol Environ Saf
February 2025
Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China; Central Laboratory of Nanjing Medical University Affiliated Nanjing Hospital, Nanjing, China; School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China. Electronic address:
Background: Perfluorooctanoic acid (PFOA) exposure has been implicated in various health issues. This study aims to identify common genes associated with PFOA exposure and ovarian cancer, elucidate their biological functions, and explore their prognostic significance.
Methods: We identified common genes linked to PFOA exposure and ovarian cancer using the Comparative Toxicogenomics Database.
GPX3 as a Novel and Potential Therapeutic Target in the Shared Molecular Mechanisms of Traumatic Brain Injury and Parkinson's Disease.
J Inflamm Res
February 2025
Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.
Background: Traumatic brain injury (TBI) is a prevalent neurological disorder associated with significant public health burdens and long-term risks, including neurodegenerative diseases such as Parkinson's disease (PD). Emerging evidence suggests a strong link between moderate to severe TBI and an elevated risk of PD, though the underlying mechanisms remain poorly understood.
Materials And Methods: Common differentially expressed genes (DEGs) were identified in GEO datasets of patients with traumatic brain injury (TBI) and Parkinson's disease (PD).
Toxicogenomic signatures of estrogen-related modes of action in the zebrafish embryo.
Environ Toxicol Chem
January 2025
Department Ecotoxicogenomics, Fraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, Germany.
Endocrine disruptors represent a diverse array of chemicals known to interfere with the endocrine systems of both human and environmental organisms, adversely affecting reproduction, development, and behavior, thus raising significant health and ecological concerns. Traditional regulatory tests for endocrine activity typically involve juvenile or adult fish, which is both time-consuming and resource-intensive and necessitates substantial animal use. This study adopts a transcriptomic approach to identify toxicogenomic signatures associated with the disruption of estrogen signaling in zebrafish (Danio rerio) embryos.
View Article and Find Full Text PDFC3AR1 as a target for preeclampsia: from bioinformatics and network pharmacology to experimental validation.
BMC Pregnancy Childbirth
January 2025
Obstetrics and Gynecology Center, Department of Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510280, China.
Background: Preeclampsia, characterized by hypertension and proteinuria during pregnancy, poses significant risks to both mother and fetus. The complement system's aberrant activation, notably the C3AR1, is important to the pathogenesis of preeclampsia, although the precise mechanisms are not fully understood.
Materials And Methods: Utilizing the Comparative Toxicogenomics Database (CTD) and Molecular Signatures Database (MSigDB), we identified complement system targets associated with preeclampsia and environmental pollutants.
Using transcriptomic signatures to elucidate individual and mixture effects of inorganic arsenic and manganese in human placental trophoblast HTR-8/SVneo cells.
Toxicol Sci
February 2025
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC 27599, United States.
Prenatal exposure to the toxic metal inorganic arsenic (iAs) is associated with adverse pregnancy and fetal growth outcomes. These adverse outcomes are tied to physiological disruptions in the placenta. Although iAs co-occurs in the environment with other metals such as manganese (Mn), there is a gap in the knowledge of the effects of metal mixtures on the placenta.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!