Modulation of plant carbon and nitrogen metabolism by novel actinobacteria Rhodospirillum sp. to combat galaxolide toxicity in barley and maize plants.

The phytotoxic effect of cosmetics such as galaxolide (HHCB) has been investigated, however, their metabolic basis of this impact is still obscure. Thus, we investigated the effect of HHCB on the biomass accumulation, photosynthesis, primary and secondary metabolites in two species from different functional groups i.e., barley (C3) and maize (C4). In addition, the metabolic bases of HHCB stress mitigating impact of the bioactive Rhodospirillum sp. JY3 were investigated. HHCB toxicity on plant growth and physiology was significantly reduced in PGPB treated plants. At metabolism level, sugars levels and metabolic enzymes (e.g., invertase, sucrose synthase, starch synthase) were increased. Consequentially, this provided a route for organic, amino and fatty acids biosynthesis. PGPB further mitigated the phytotoxic impact of HHCB upon the levels of organic acids (e.g., oxalic, citric, succinic, malic and isobutyric acids), amino acids, particularly proline, in addition to unsaturated fatty acids. Furthermore, plant growth-promoting bacteria (PGPB) treatment reduced HHCB toxicity through increasing antioxidant metabolites (e.g., polyamines and anthocyanin), their precursors (e.g., phenylalanine, naringenin, cinnamic and coumaric acids) and their related biosynthetic enzymes such as chalcone synthase and cinnamate-4-hydroxylase. Overall, this study, for the first time, significantly contributes to quenching the environmental hazards and maintaining agriculture sustainability using eco-friendly tools.