Silk-based biomaterials for tissue engineering.

Tissue engineering (TE) involves repairing, replacing, regeneration, or improving the function of tissues and organs by combining cells, growth factors and scaffold materials. Among these, scaffold materials play a crucial role. Silk fibroin (SF), a natural biopolymer, has been widely used in the TE field due to its good biodegradability, biocompatibility, and mechanical properties attributed to its chemical composition and structure. This paper reviews the structure, extraction, and modification methods of SF. In addition, it discusses SF's regulation of cell behavior and its various processing modes. Finally, the applications of SF in TE and perspectives on future developments are presented. This review provides comprehensive and alternative rational insights for further biomedical translation in SF medical device design, further revealing the great potential of SF biomaterials.