sp. nov., isolated from the faecal sample of a zoo animal, .

Int J Syst Evol Microbiol

Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.

Published: January 2025

Strain NoAH (=KACC 23135=JCM 35999), a novel Gram-negative, motile bacterium with a rod-shaped morphology, was isolated from the zoo animal faecal samples, specifically the long-tailed goral species . The novel bacterial strain grew optimally in a nutrient broth medium under the following conditions: 1-2% (w/v) NaCl, pH 7-8 and 30 °C. The strain NoAH exhibited high tolerance to NaCl, with the ability to tolerate up to 7% (w/v) NaCl. Based on phylogenetic analyses using 16S rRNA gene sequencing, strain NoAH was found to have the closest relatedness to YW1 (98.5%), ATCC 11330 (97.9%), KCTC 82561 (97.9%), CJ34 (97.7%) and EJ-4 (97.6%). The genome size and genomic DNA G+C content of strain NoAH were 4.05 Mbp and 55.9 mol%, respectively. A whole-genome-level comparison of strain NoAH with YW, LMG 3475, NBRC 14918, NBRC 12685 and CJ34 revealed the following orthologous average nucleotide identity values: 80.1, 79.0, 78.6, 76.3 and 75.2%, respectively. The major polar lipids of strain NoAH were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Considering our findings in chemotaxonomic, genotypic and phenotypic characteristics, strain NoAH is identified as a novel species within the genus , for which the name sp. nov. is proposed.

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http://dx.doi.org/10.1099/ijsem.0.006665DOI Listing

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