In this paper, we introduce a new method for precise determination of the calibration function of liquid crystal spatial light modulators (LC-SLMs). The proposed method shares the advantages of commonly used methods, interferometric and diffraction-based methods, and is free of their disadvantages. The method proposed here is based on the evaluation of Fresnel diffraction of light from a phase-discontinuity pattern loaded into the SLM device. In one version, the phase step pattern consists of two parts, one remains constant at zero, while the other changes with steps of 5 from 0 to 255 gray levels, for each measurement. By fitting the experimental intensity distribution measured in this configuration on the corresponding theoretical intensity distribution, complete phase calibration can be obtained. We also propose a second version, employing a phase wedge pattern, allowing the phase calibration curve of the SLM to be determined by just a single frame measurement which is a great asset.
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http://dx.doi.org/10.1364/OE.553970 | DOI Listing |
Nat Biotechnol
January 2025
Department of Automation, Tsinghua University, Beijing, China.
Super-resolution (SR) neural networks transform low-resolution optical microscopy images into SR images. Application of single-image SR (SISR) methods to long-term imaging has not exploited the temporal dependencies between neighboring frames and has been subject to inference uncertainty that is difficult to quantify. Here, by building a large-scale fluorescence microscopy dataset and evaluating the propagation and alignment components of neural network models, we devise a deformable phase-space alignment (DPA) time-lapse image SR (TISR) neural network.
View Article and Find Full Text PDFIn this paper, we introduce a new method for precise determination of the calibration function of liquid crystal spatial light modulators (LC-SLMs). The proposed method shares the advantages of commonly used methods, interferometric and diffraction-based methods, and is free of their disadvantages. The method proposed here is based on the evaluation of Fresnel diffraction of light from a phase-discontinuity pattern loaded into the SLM device.
View Article and Find Full Text PDFHolographic light potentials generated by phase-modulating liquid-crystal spatial light modulators (SLMs) are widely used in quantum technology applications. Accurate calibration of the wavefront and intensity profile of the laser beam at the SLM display is key to the high fidelity of holographic potentials. Here, we present a new calibration technique that is faster than previous methods while maintaining the same level of accuracy.
View Article and Find Full Text PDFClin Pharmacol Ther
January 2025
Leiden Experts on Advanced Pharmacokinetics and Pharmacodynamics (LAP&P), Leiden, The Netherlands.
Daprodustat, a novel oral hypoxia-inducible factor prolyl hydroxylase inhibitor is approved in the United States for the treatment of anemia due to chronic kidney disease (CKD) in adults receiving dialysis for at least 4 months. Pharmacodynamic dose-hemoglobin (Dose-Hgb) models were developed as daprodustat progressed through development. To support global phase III development, a dose-titration algorithm, guided by simulations from the initial Dose-Hgb model based on phase II clinical data, was implemented.
View Article and Find Full Text PDFJ Biol Eng
January 2025
AVT - Biochemical Engineering, RWTH Aachen University, Forckenbeckstr. 51, D-52074, Aachen, Germany.
Background: Shake flasks are essential tools in biotechnological development due to their cost efficiency and ease of use. However, a significant challenge is the miniaturization of process analytical tools to maximize information output from each cultivation. This study aimed to develop a respiration activity online measurement system via off-gas analysis, named "Transfer rate Online Measurement" (TOM), for determining the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and the respiration quotient (RQ) in surface-aerated bioreactors, primarily targeting shake flasks.
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