Potential of lavender essential oil to inhibit tetracycline resistance and modulate gut microbiota in black soldier fly larvae.

The misuse of tetracycline in livestock farming leads to environmental residues that promote the proliferation of antibiotic resistance genes (ARGs), particularly tetracycline resistance (tet) genes. Black soldier fly (BSF) larvae, used for organic waste bioconversion, may carry tetracycline residues in their guts, raising concerns about ARG spread. To address this issue, plant-derived additives such as lavender essential oil (LEO) have been explored as alternative antibiotics. This study investigated the effects of LEO on tet gene suppression and gut microbiota modulation in BSF larvae. Results showed that oxytetracycline treatment increased tet gene relative abundance threefold compared to the control, reaching 1.13 ± 0.29 and enriched pathogens Klebsiella oxytoca and Enterobacter hormaechei. Conversely, LEO treatment (100 mg/kg) reduced tet gene abundance by 46.67 %, from 0.15 ± 0.02 to 0.08 ± 0.02, and enhanced beneficial microorganisms like Leuconostoc pseudomesenteroides. Furthermore, LEO reduced tet gene relative abundance in oxytetracycline-treated larvae from 1.13 ± 0.29 to 0.49 ± 0.19 and 0.70 ± 0.11 in separate treatments. LEO modified fungal composition and nutrient pathways. Network analysis revealed that LEO promoted a more integrated and modular gut microbiota, enhancing functional specialization and resilience. These findings suggest LEO can mitigate ARGs in BSF larvae, offering a sustainable approach for antibiotic resistance management in organic waste recycling and livestock farming.