Dihydroaurones and Isoflavan Derivatives from the Roots of .

As a continuation of our pharmacognostic studies on different species growing in Türkiye, the phytochemical investigation of the roots of Hub.-Mor., a licorice species endemic to Türkiye, was carried out. This study yielded twenty-three secondary metabolites (-) including nine previously unreported compounds: two dihydroaurone-3-enoic acids, licoaurone A () and licoaurone B (), isoflavan hydroxypreglabridin (), isoflavanone cyclodeoxykievitone (), flavanone-3-ol glycyasymmetrol (), and four bi-isoflavans, glycyasymmetrica A-D (-). The structures of isolated compounds were established by NMR and MS experiments. The relative configurations (-) were assigned by a combined quantum mechanical/NMR approach, comparing the experimental C/H NMR chemical shift data and the related predicted values. The absolute configurations of compounds - were assigned by comparison of their experimental electronic circular dichroism curves with the TDDFT-predicted curves. All isolated compounds were also evaluated for their cytotoxic activity against MCF-7, HeLa, HepG2, and A549 cancer cell lines by using MTT assay.