Background: Spinach ( L.) is an important leafy vegetable with dioecious and occasional monoecious plants. Monoecious lines are more suitable for hybrid production than dioecious lines due to their extended flowering period. However, genetic research on the sex determination of monoecism remains limited.
Methods: In this study, RNA-seq analysis of monoecious and female spinach plants was performed at two distinct flowering stages. In total, we identified 4586 differentially expressed genes (DEGs), which were primarily involved in biological processes such as hormone signaling, cell wall biosynthesis, photosynthesis, and flower development, based on Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Results: Among these DEGs, 354 transcription factors, including 27 genes associated with the ABCDE gene, were discovered. Furthermore, a co-expression gene regulatory network was built, identifying nine key genes that play important roles in regulating sex differentiation between female and monoecious plants.
Conclusions: Our findings provide crucial molecular insights into the mechanisms of monoecism in spinach and offer a scientific basis for future spinach breeding.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3390/genes16010024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative Transcriptome Analysis of Gene Expression Between Female and Monoecious L.
Genes (Basel)
December 2024
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Background: Spinach ( L.) is an important leafy vegetable with dioecious and occasional monoecious plants. Monoecious lines are more suitable for hybrid production than dioecious lines due to their extended flowering period.
View Article and Find Full Text PDFThe calcium-dependent protein kinase CmaCPK4 regulates sex determination in pumpkin (Cucurbita maxima D.).
Plant Physiol
December 2024
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/Northeast Agricultural University, Harbin, 150030, China.
Pumpkin (Cucurbita maxima D.) is typically monoecious with individual male and female flowers, and its yield is associated with the degree of femaleness, i.e.
View Article and Find Full Text PDF (Asteraceae), a new species from Xizang (Tibet), China.
PhytoKeys
November 2024
The Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Xizang Characteristic Agricultural and Animal Husbandry Resources, The Center for Xizang Chinese (Tibetan) Medicine Resources, Joint Laboratory for Tibetan Materia Medica Resources Scientific Protection and Utilization Research of Tibetan Medical Research Center of Xizang, Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Tibet Autonomous Region, Xizang Agriculture and Animal Husbandry University, Nyingchi, Xizang 860000, China Xizang Agriculture and Animal Husbandry University Nyingchi China.
, a new species of Asteraceae from the Xizang (Tibet) Autonomous Region of China, is described and illustrated here. Morphologically, it is most similar to but can be distinguished by the combination of monoecious and dioecious individuals, involucral bracts arranged in 3-5 series (with outer series herbaceous and middle to inner series membranous), fimbriate apices on female florets, rough-edged lobes on male florets, and achenes lacking costae. Phylogenetic analyses further support the separation of this new species from related taxa.
View Article and Find Full Text PDFNegative and sex-specific effects of drought on flower production, resources and pollinator visitation, but not on floral scent in monoecious Cucurbita pepo.
New Phytol
November 2024
Department of Environment and Biodiversity, Paris Lodron University of Salzburg, Hellbrunnerstrasse 34, Salzburg, 5020, Austria.
The globally changing climatic condition is increasing the incidences of drought in several parts of the world. This is predicted and already shown to not only impact plant growth and flower development, but also plant-pollinator interactions and the pollination success of entomophilous plants. However, there is a large gap in our understanding of how drought affects the different flowers and pollen transfer among flowers in sexually polymorphic species.
View Article and Find Full Text PDFThe transcription factor CpMYB62 controls the genetic network that leads to the determination of female flowers in .
Hortic Res
June 2024
Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120 Almería, Spain.
In monoecious species, female flowering constitutes the developmental process that determines the onset and production of fruit and is therefore closely related to crop yield. This article presents the identification and phenotypic and molecular characterization of , an ethylmethane sulfonate loss-of-function mutation that completely blocks the female floral transition, converting all female flowers into male flowers. BSA-seq analysis coupled with WGS showed that corresponds to a C>T transition in the coding region of the gene , generating a premature stop codon and a truncated transcription factor without its N-terminal effector domain.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!