is a protogynous hermaphroditic fish that changes from female to male, but the underlying sex change mechanism remains as-yet unknown. In this study, we firstly cloned and characterized the sequence and protein structure of of We found that the genomic structure of was different from other species. Expression was detected in the developing gonad by applying qRT-PCR and in situ hybridization. We found that the expression of in the ovotestis was higher than in the ovary and testes. A strong signal of was detected in oocytes and granulosa cells in the ovary and spermatogonia and primary spermatocytes in the testes. We found that the promoter methylation of was negatively correlated with gene expression in developing gonads, especially at site 114. A dual-luciferase assay was designed and revealed that regulates promoter activity opposite to . In summary, during sex reversal, DNA methylation affects the binding of the transcription factor dmrt1 and in the promoter region through methylation and demethylation interactions to regulate the expression of during gonadal development.
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