One single subcutaneous injection of 100 micrograms protein of a glycoprotein fraction from UV light-induced mouse sarcoma produces transplantation resistance against the same tumor. The glycoprotein fraction was isolated by 2% Triton X-100 and 3M KCl from enriched membranes. As measured by immunogenicity a tenfold enrichment of a tumor-associated antigen was obtained when compared with the 3M KCl extract on the basis of protein concentration.
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