[Knockdown of promotes osteogenic differentiation of human bone marrow mesenchymal stem cells].

Objective: To initially investigate the function of neuronal pentraxin 1 () gene on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).

Methods: hBMSCs were induced to undergo osteogenic differentiation, and then RNA was collected at different time points, namely 0, 3, 7, 10 and 14 d. The mRNA expression levels of key genes related with osteogenic differentiation, including runt-related transcription factor 2 (), alkaline phosphatase (), osteocalcin (), and , were detected on the basis of quantitative real-time polymerase chain reaction (qPCR) technology. In order to establish a stable -knockdown hBMSCs cell line, shRNA lentivirus was constructed and used to infect hBMSCs. ALP staining, alizarin red (AR) staining, and qPCR were employed to assess the impact of -knockdown on the osteogenic differentiation ability of hBMSCs.

Results: The results showed that during the osteogenic differentiation of hBMSCs , the mRNA expression levels of osteogenic genes , and significantly increased compared with 0 d, while expression decreased markedly ( < 0.01) as the osteogenic induction period exten-ded. At 72 h post-infection with lentivirus, the result of qPCR indicated that the knockdown efficiency of was over 60%. After knocking down in hBMSCs, RNA was extracted from both the -knockdown group (sh group) and the control group (shNC group) cultured in regular proliferation medium. The results of qPCR showed that the expression levels of osteogenic-related genes and osterix () were significantly higher in the sh group compared with the shNC group ( < 0.01). ALP staining revealed a significantly deeper coloration in the sh group than in the shNC group at the end of 7 d of osteogenic induction. AR staining demonstrated a marked increase in mineralized nodules in the sh group compared with the shNC group at the end of 14 d of osteogenic induction.

Conclusion: exerts a modulatory role in the osteogenic differentiation of hBMSCs, and its knockdown has been found to enhance the osteogenic differentiation of hBMSCs. This finding implies that could potentially serve as a therapeutic target for the treatment of osteogenic abnormalities, including osteoporosis.