Chromatin Regulation of Acetic Acid Stress Tolerance by Ino80 in Budding Yeast .

Enhanced environmental stress tolerance is important for microbial production of biofuels and biobased chemicals. However, the roles of chromatin regulation in stress tolerance and bioproduction remain unclear. Here, we explore the effects of Ino80, the core subunit of the INO80 chromatin remodeling complex, on yeast stress adaptation. We found that deletion of led to increased sensitivity of budding yeast to acetic acid, which is a common inhibitor in lignocellulosic biomass and also serves as a food preservative. Integrated ATAC-seq and RNA-seq analyses further showed that deleting resulted in extensive changes in chromatin accessibility and gene expression in cell wall-related genes. Genetic interaction between and , which is a subunit of the ADA acetyltransferase complex was proved, and the direct role of in the expression of transcription was also confirmed. These findings benefit the development of robust yeast strains and food preservatives by targeting chromatin regulation.