This study explored the effect of electrical stimulation (ES) and Pediococcus pentosaceus LL-07 (P. pentosaceus LL-07) and Staphylococcus simulans QB7 (S. simulans QB7) on the quality and microbial community of loin ham during the ripening. After the ES and starter culture treatments, the Aw and pH were decreased. Surface hydrophobicity, myogenic fiber fragmentation index (MFI), TCA-soluble peptide, amino nitrogen and free amino acids (FAAs) were also significantly higher than the control group (CK) (P < 0.05). This increase was more significant in the E-S group (electrical stimulation followed by inoculation with P. pentosaceus LL-07 and S. simulans QB7) than the rest of the experimental group (E、S、S-E group). Furthermore, the CK and E-S groups were subjected to a bacterial community comparison experiment. The microbial diversity of these two groups was increased. Pediococcus spp. and Staphylococcus spp. became the dominant bacteria in E-S groups during the ripening. Correlation analyses show a strong correlation between protein hydrolysis, microorganisms and FAAs. In conclusion, the combination of ES and starter culture could promote protein hydrolysis, the accumulation of FAAS, and improves the bacterial community of loin ham.
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http://dx.doi.org/10.1016/j.foodres.2024.115640 | DOI Listing |
Food Res Int
February 2025
College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address:
Effects of enzymolysis by seven proteases (Alcalase, Bromelain, Flavourzyme, Papain, Pepsin, Protamex, and Trypsin) with distinct cleavage specificities on the emulsification performance of hempseed protein (HPI) and its correlation with the structural and interfacial characteristics were explored in this study. Upon enzymolysis, a remarkable decrease in α-helix and β-turn was observed in resultant hydrolysates (HPH), accompanied by a rise in β-sheet and random coil, notably by Alcalase, Bromelain, Papain, and Trypsin. Overall, proteolysis led to noticeable reductions in surface hydrophobicity and total sulfhydryls as well as a redshift in intrinsic fluorescence, with Papain showing the most pronounced effects, possibly due to its higher hydrolysis degree (4.
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February 2025
School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; Key Laboratory of Agricultural and Animal Products Store and Processing of Guizhou Province, Guiyang 550025, China. Electronic address:
This study explored the effect of electrical stimulation (ES) and Pediococcus pentosaceus LL-07 (P. pentosaceus LL-07) and Staphylococcus simulans QB7 (S. simulans QB7) on the quality and microbial community of loin ham during the ripening.
View Article and Find Full Text PDFFood Res Int
February 2025
Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark. Electronic address:
Currently, little is known about how complex carbohydrates (maltodextrins) with varying degrees of polymerisation (DP) and molecular branching interact with α-amylase in human saliva and the associated amounts and structures of generated reducing sugars. Therefore, this study aimed to investigate salivary α-amylase and the subsequent reducing sugars generated with complex carbohydrate stimuli. A secondary aim was to investigate reducing sugar generation and complex carbohydrate taste sensitivity.
View Article and Find Full Text PDFFood Res Int
February 2025
Food Science and Nutrition Department, University of Minnesota, 1334 Eckles Ave, Saint Paul, MN, 55108, United States. Electronic address:
There is an ever-increasing demand for novel plant proteins that are non-allergenic, nutritionally complete, adequately functional, and can be sustainably sourced. RuBisCo is a protein that fulfills these requirements and can be sourced from alfalfa (Medicago sativa). Therefore, this study investigated several techniques to adequately extract alfalfa protein.
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February 2025
Department of Food Technology, Federal University of Viçosa, Av. Peter Henry Rolfs, S/n, University Campus, 36570-900, Viçosa, MG, Brazil. Electronic address:
Adding value to food by-products, such as pumpkin seeds, is an important strategy for the complete utilization of plant foods and advancing sustainability goals. This study aimed to maximize the production of bioactive peptides from pumpkin seed protein (PSP) by combining ultrasonic (US) pretreatment (40 kHz, 23.8 W/L) with enzymatic hydrolysis.
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