Studies presenting visible-light-induced desulfurization of peptides containing a cysteine residue have been carried out. This transformation driven by light-emitting-diode-type light proceeds with high efficiency in an aqueous solution at room temperature and involves the use of a catalytic amount of photosensitizer, Rose Bengal. The procedure has been tested on model synthetic peptides, lysozyme C and α-crystallin, and successfully applied to a one-pot native chemical ligation (NCL)-desulfurization protocol.
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Shining a Light on Peptide and Protein Synthesis: Light-Emitting-Diode-Driven Desulfurization of Cysteine to Alanine with Rose Bengal.
Org Lett
January 2025
Faculty of Chemistry, University of Wrocław, Joliot-Curie 14 Street, 50-383 Wrocław, Poland.
Studies presenting visible-light-induced desulfurization of peptides containing a cysteine residue have been carried out. This transformation driven by light-emitting-diode-type light proceeds with high efficiency in an aqueous solution at room temperature and involves the use of a catalytic amount of photosensitizer, Rose Bengal. The procedure has been tested on model synthetic peptides, lysozyme C and α-crystallin, and successfully applied to a one-pot native chemical ligation (NCL)-desulfurization protocol.
View Article and Find Full Text PDFCamel Brucellosis: Seroprevalence, Associated Risk Factor, and Public Health Perceptions in Arero District of Borena Zone, Southern Ethiopia.
Vet Med Int
January 2025
Faculty of Veterinary Medicine, Hawassa University, P.O. Box 05, Hawassa, Ethiopia.
A cross-sectional study was conducted to determine the seroprevalence and potential risk factors of camel brucellosis and to assess public health awareness of the disease in the selected kebele of Arero District, Borena Zone, Southern Ethiopia. A total of 313 blood samples were collected from selected camels using a systematic random sampling technique. The serum samples underwent initial screening for brucellosis using the rose Bengal plate test (RBPT), with further confirmation through the indirect enzyme-linked immunosorbent Assay (i-ELISA).
View Article and Find Full Text PDFPhotosensitizable ZIF-8 BioMOF for Stimuli-Responsive Antimicrobial Phototherapy.
Mol Pharm
January 2025
Department of Nanomaterials and Application Technology, Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Government of India, Sector 81 (Knowledge City), S.A.S. Nagar, Mohali 140306, Punjab, India.
Resistant pathogens are increasingly posing a heightened risk to healthcare systems, leading to a growing concern due to the lack of effective antimicrobial treatments. This has prompted the adoption of antimicrobial photodynamic therapy (aPDT), which eradicates microorganisms by generating reactive oxygen species (ROS) through the utilization of a photosensitizer, photons, and molecular oxygen. However, a challenge arises from the inherent characteristics of photosensitizers, including photobleaching, aggregation, and self-quenching.
View Article and Find Full Text PDF[Knowledge, practices, and human seroprevalence of brucellosis in the central dairy-producing area of Cochabamba, BoliviaConhecimentos, práticas e soroprevalência humana de brucelose na área central de produção de laticínios de Cochabamba, Bolívia].
Rev Panam Salud Publica
January 2025
Departamento de zootecnia y producción animal Facultad de Ciencias Agrícolas Pecuarias y Forestales, Universidad Mayor de San Simón Cochabamba Estado Plurinacional de Bolivia Departamento de zootecnia y producción animal, Facultad de Ciencias Agrícolas, Pecuarias y Forestales, Universidad Mayor de San Simón, Cochabamba, Estado Plurinacional de Bolivia.
Objective: To validate the applicability of the Rose Bengal (RB) test, indirect IgG or IgM enzyme-linked immunosorbent assay (ELISA), and the Brucellacapt test to the local context, determine the seroprevalence of brucellosis, and evaluate knowledge of the disease in people at risk in a dairy-producing area of La Maica, Cochabamba (Plurinational State of Bolivia).
Method: The tests were initially validated with a group of 76 people; the final study population was 330 people, who also participated in a socio-epidemiological survey.
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Immunological and protective evaluation of purE/purK gene-deletion mutant of Brucella melitensis M5 strain.
Microb Pathog
January 2025
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Yujinxiang Street 573, Changchun, Jilin, 130122, PR China. Electronic address:
Brucellosis is listed by the World Health Organization as one of the seven most neglected global zoonotic diseases. A live-attenuated vaccine is still the main strategy used to prevent the spread of brucellosis. In this study, we constructed a two-gene (purE and purK)-deletion vaccine in Brucella melitensis vaccine strain M5 with homologous recombination.
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