With the US Environment Protection Agency reducing requests for (and funding of) mammalian studies alongside the proposed elimination of requests by 2035, there is an urgent need for fully validated New Approach Methods (NAMs) to fill the resultant gap for safety assessment of agrochemicals. One promising NAM for assessing the potential for human prenatal developmental toxicity potential is the Zebrafish Embryo Developmental Toxicity Assessment, a bioassay that has been used by the pharmaceutical industry for more than a decade in early-stage drug safety assessment. Despite its promise, little data has been generated to assess the validity of ZEDTA for assessing Developmental and Reproductive Toxicity of new agrochemical products. Addressing this knowledge gap, we tested 67 compounds (insecticides, herbicides and fungicides) spanning multiple different chemical groupings and mechanisms of action. ZEDTA assay results were compared with the European Chemicals Agency (ECHA) Classification and Labelling (C&L) for mammalian hazard classification and with publicly available data to determine the ZEDTA's translation power. Overall, the ZEDTA assay had an effective detection capability of 65% for sensitivity and 64% for specificity as compared with the ECHA-C&L classification and publicly available data. Comparing the ZEDTA data there were both strengths and weaknesses in alignments for across the different chemical classes and chemical mechanisms of action. Overall, the data generated, show the performance of the ZEDTA assay was comparable with other bioassays highlighted as alternatives for mammalian assessment and holds good promise as a NAM for screening agrochemical prenatal developmental toxicity during new product human safety assessment.
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http://dx.doi.org/10.1016/j.reprotox.2025.108837 | DOI Listing |
Reprod Toxicol
January 2025
Biosciences, Faculty of Health and Life Sciences, University of Exeter, Stocker Road, Exeter, Devon, EX4 4QD, UK. Electronic address:
With the US Environment Protection Agency reducing requests for (and funding of) mammalian studies alongside the proposed elimination of requests by 2035, there is an urgent need for fully validated New Approach Methods (NAMs) to fill the resultant gap for safety assessment of agrochemicals. One promising NAM for assessing the potential for human prenatal developmental toxicity potential is the Zebrafish Embryo Developmental Toxicity Assessment, a bioassay that has been used by the pharmaceutical industry for more than a decade in early-stage drug safety assessment. Despite its promise, little data has been generated to assess the validity of ZEDTA for assessing Developmental and Reproductive Toxicity of new agrochemical products.
View Article and Find Full Text PDFEcotoxicol Environ Saf
January 2025
Fisheries College, Huazhong Agricultural University, Wuhan 430070, China; Hubei Engineering Technology Research Center for Aquatic Animal Disease Control and Prevention, Wuhan 430070, China. Electronic address:
The toxicity of tris (2-butoxyethyl) phosphate (TBOEP) has been extensively investigated because of its prevalence in the environment. Nevertheless, the risk factors associated with maternal transmission are poorly understood. In this study, sexually mature female zebrafish were treated with TBOEP (0, 20, 100, and 500 μg/L) for 30 days and were mated with unexposed males.
View Article and Find Full Text PDFNaunyn Schmiedebergs Arch Pharmacol
January 2025
Department of Biochemistry, University of Ilorin, Kwara State, Ilorin, Nigeria.
This study carried out a quantitative structure-activity relationship hazard assessment of the banned pesticides in Nigeria with a view of identifying the dangers posed by these pesticides. Structure-activity relationships (SARs) and quantitative structure-activity relationships (QSARs), which link a compound's chemical structure to its biological activity, can be used to create safer and more effective insecticides, prioritize chemicals for testing, and reduce the number of animal studies necessary throughout the regulatory process. The QSAR hazard assessment of the banned pesticides was carried out on the VEGA software.
View Article and Find Full Text PDFJ Xenobiot
January 2025
Laboratoire de Biologie du Développement (LBDV), Institut de la Mer de Villefranche (IMEV), Sorbonne Université, Centre National de la Recherche Scientifique (CNRS), 06230 Villefranche-sur-Mer, France.
Nanoplastics pose a growing threat to marine ecosystems, particularly affecting the early developmental stages of marine organisms. This study investigates the effects of amino-modified polystyrene nanoparticles (PS-NH, 50 nm) on the embryonic development of , a model ascidian species. Both chorionated and dechorionated embryos were exposed to increasing concentrations of PS-NH so morphological alterations could be assessed with a high-content analysis of the phenotypes and genotoxicity.
View Article and Find Full Text PDFSe Pu
February 2025
College of Chemical Engineering and Environment, China University of Petroleum-Beijing, Beijing 102249, China.
Trace contaminants are toxic and their widespread presence in the environment potentially threatens human health. The levels of these pollutants are often difficult to determine directly using instruments owing to the complexities of environment matrices. Hence, pretreatment steps, such as sample purification and concentration, are key along with various processes that enhance the accuracy and sensitivity of the detection method.
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