The application of chitin materials in enzymatic catalysis: A review.

Enzymatic catalysis offers notable advantages, including exceptional catalytic efficiency, selectivity, and the ability to operate under mild conditions. However, its widespread application is hindered by the high costs associated with enzymes and cofactors. Materials-mediated immobilization technology has proven effective in the recycling of enzymes and cofactors. An optimal carrier material for protein immobilization must be non-toxic, biocompatible, and should not compromise the biological activity or structure of the enzymes. Compared to synthetic polymers, chitin is a promising carrier given its low cost, renewability, abundance of functional groups, and notable biocompatibility and biodegradability. Although numerous reviews on chitosan and other polymers for immobilization have been published, few have addressed using chitin as supports. In this review, chitin-based materials mediated enzyme immobilization, the one-step purification and immobilization of enzymes, as well as co-immobilization of enzymes and cofactors were summarized. Particularly, the significance of chitin materials in the field of enzymatic catalysis was emphasized. This study has the potential to open new avenues for immobilized biocatalysts.