Effect of resveratrol on post-thaw motility, kinematics, structural parameters and antioxidant/oxidant status of Kamori buck spermatozoa.

Resveratrol is a polyphenol compound showing strong antioxidant properties. It is believed that semen cryopreservation causes significant sperm losses which eventually affects sperm quality. Improving antioxidant status of semen may reduce this damage and enhance sperm fertilizing potential. This study investigated the resveratrol treated freeze-thawing sperm motion, kinetics, structural parameters and antioxidant/oxidant status of Kamori buck spermatozoa. Thirty-two ejaculates from 4 fertile Kamori bucks were processed in tris-citric-glyercol-egg yolk (TCG-EY) based with varying concentrations of resveratrol (0, 10, 20, 40 and 50 μM). Over 75% sperm motility were pooled and frozen in liquid nitrogen. The results unveiled that adding resveratrol at 40 and 50 μM concentration significantly enhanced (P < 0.05) total motility (progressive motility, rapid velocity, and average path velocity, straight line velocity, curvilinear velocity, amplitude of lateral head displacement, beat cross frequency, straightness and linearity in contrast with control and other groups. Supplementing resveratrol at 40 and 50 μM concentration significantly improved (P < 0.05) functional plasma membrane integrity, acrosome integrity, whereas, all resveratrol groups had same significant (P < 0.05) effect on DNA integrity in response to control group. The 40 and 50 μM resveratrol significantly promoted (P < 0.05) superoxide dismutase (SOD), catalase (CAT), peroxidases (POD), and total antioxidant capacity (TAC) levels while significantly reducing (P < 0.05) the total oxidant status (TOS) and malondialdehyde (MDA) contents as compared to control and other groups, respectively. The principal component analysis (PCA) exhibited samples were present in different clusters except two groups which had partially overlapped. Using hierarchical clustering analysis, two clusters were constructed showing the relationship within the treated groups. The results of spearman correlation coefficient showed that yellow color showed highly positive correlation while turquoise color exhibited highly negative association between sperm variables. Overall, the results concluded that resveratrol at 50 μM performed slightly better results than 40 μM in terms of improving sperm quality parameters.