Lanthanide(III) complexes with two-photon absorbing antennas are attractive for microscopy imaging of live cells because they can be excited in the NIR. We describe the synthesis and luminescence and imaging properties of two Eu complexes, and , with (-carbazolyl)-aryl-alkynyl-picolinamide and (-carbazolyl)-aryl-picolinamide antennas, respectively, conjugated to the TAT cell-penetrating peptides. Contrary to what was previously observed with related Eu complexes with carbazole-based antennas in a mixture of water and organic solvents, these two complexes show very low emission quantum yield (Φ < 0.002) in purely aqueous buffers. A detailed spectroscopic study on reveals that the quantum yield of emission is strongly polarity dependent─the less polar the medium, the higher the quantum yield─and that the emission quenching in water is likely due to a photoinduced electron transfer between the excited carbazole-based antenna and Eu that efficiently competes with the energy transfer process. Nevertheless, shows a significant two-photon cross-section of 100 GM at 750 nm, which is interesting for two-photon microscopy. The live cell imaging properties of and the two other conjugates were investigated. Cytosolic delivery was clearly evidenced in the case of when cells are coincubated with this compound and a nonluminescent dimeric TAT derivative, dFFLIPTAT.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.inorgchem.4c04806 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In vivo two-photon FLIM resolves photosynthetic properties of maize bundle sheath cells.
Photosynth Res
January 2025
State Key Laboratory of Forage Breeding-by-Design and Utilization, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Maize (Zea mays L.) performs highly efficient C photosynthesis by dividing photosynthetic metabolism between mesophyll and bundle sheath cells. In vivo physiological measurements are indispensable for C photosynthesis research as photosynthetic activities are easily interrupted by leaf section or cell isolation.
View Article and Find Full Text PDFCarbazole-Based Eu Complexes for Two-Photon Microscopy Imaging of Live Cells.
Inorg Chem
January 2025
Univ. Grenoble Alpes, CNRS, CEA, IRIG, LCBM (UMR 5249), Grenoble F-38000, France.
Lanthanide(III) complexes with two-photon absorbing antennas are attractive for microscopy imaging of live cells because they can be excited in the NIR. We describe the synthesis and luminescence and imaging properties of two Eu complexes, and , with (-carbazolyl)-aryl-alkynyl-picolinamide and (-carbazolyl)-aryl-picolinamide antennas, respectively, conjugated to the TAT cell-penetrating peptides. Contrary to what was previously observed with related Eu complexes with carbazole-based antennas in a mixture of water and organic solvents, these two complexes show very low emission quantum yield (Φ < 0.
View Article and Find Full Text PDFUnusual Iron-Independent Ferroptosis-like Cell Death Induced by Photoactivation of a Typical Iridium Complex for Hypoxia Photodynamic Therapy.
ACS Appl Mater Interfaces
January 2025
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
Ferroptosis is a unique cell death mode that relies on iron and lipid peroxidation (LPO) and is extensively utilized to treat drug-resistant tumor. However, like the other antitumor model, requirement of oxygen limited its application in treating the malignant tumors in anaerobic environments, just as photodynamic therapy, a very promising anticancer therapy. Here, we show that an iridium(III) complex (Ir-dF), which was often used in proton-coupled electron transport (PCET) process, can induce efficient cell death upon photo irradiation, which can be effectively protected by the typical ferroptosis inhibitor Fer-1 but not by the classic iron chelating agents and ROS scavengers.
View Article and Find Full Text PDFPhase-dependent Hanbury-Brown and Twiss effect for the complete measurement of the complex coherence function.
Light Sci Appl
January 2025
Department of Physics, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, China.
Hanbury-Brown and Twiss (HBT) effect is the foundation for stellar intensity interferometry. However, it is a phase insensitive two-photon interference effect. Here we extend the HBT interferometer by mixing intensity-matched reference fields with the input fields before intensity correlation measurement.
View Article and Find Full Text PDFFAST: Fast, free, consistent, and unsupervised oligodendrocyte segmentation and tracking system.
eNeuro
January 2025
Penn Statistics in Imaging and Visualization Center (PennSIVE), Department of Biostatistics, Epidemiology, and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, 19104, USA.
To develop reparative therapies for neurological disorders like multiple sclerosis (MS), we need to better understand the physiology of loss and replacement of oligodendrocytes, the cells that make myelin and are the target of damage in MS. In vivo two-photon fluorescence microscopy allows direct visualization of oligodendrocytes in the intact brain of transgenic mouse models, promising a deeper understanding of the longitudinal dynamics of replacing oligodendrocytes after damage. However, the task of tracking the fate of individual oligodendrocytes requires extensive effort for manual annotation and is especially challenging in three-dimensional images.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!