Photoelectrochemical (PEC) immunosensors are highly promising tools for monitoring biochemical molecules. Constructing high-performance heterojunctions is a general method to improve the sensitivity of PEC immunosensors. The internal electric field (IEF) formed at the heterojunction interface plays a crucial role in coordinating the separation of photogenerated carriers. Although IEF in heterojunctions shows extraordinary talents in PEC immunosensors, it is powerless to suppress the recombination of photogenerated carriers in the bulk phase. Thus, modulating the IEF of heterojunctions more than just at interfaces remains a challenge. Herein, a "signal-off" PEC immunosensor for CYFRA21-1 detection was constructed. The immunosensor was based on sulfurized bismuth oxyhydroxide compound BiS/BiO(OH) nanorods as the platforms, N-doped hollow homogenous MoC nanospheres with Ru NPs (MoC:N@Ru) as the quenching label. The BiO(OH) were composed of [BiO] and OH alternating layers, which provided an IEF throughout the bulk phase. Meanwhile, the heterogeneous interface further promoted the separation of photogenerated carriers and output strong photocurrent signals by energy level matching of BiO(OH) with BiS. Subsequently, MoC:N@Ru was quantitatively introduced in the presence of CYFRA21-1 through specific recognition between antigens and antibodies. The main structure of MoC:N@Ru was a homojunction formed by cubic and hexagonal phases MoC, which accelerated the separation of photogenerated carriers. Moreover, doped N element and Ru NPs further enhanced the competition between MoC:N@Ru and the BiS/BiO(OH) platforms for ascorbic acid and then achieved a quenching effect. The limit of detection for CYFRA21-1 was 17 fg/mL, and linearity range was 50 fg/mL -100 ng/mL. This PEC immunosensor achieves highly sensitive detection of CYFRA21-1 and has good scalability, which provides new ideas for the development analysis and detection platforms of tumor biomarkers. Simultaneously, this strategy might provide a novel perspective for modulating the distribution of the IEF of photoactive materials applied in PEC immunosensors.
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http://dx.doi.org/10.1016/j.aca.2024.343591 | DOI Listing |
Anal Chim Acta
February 2025
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, Jinan, PR China; Department of Chemistry, Sungkyunkwan University, 16419, Suwon, Republic of Korea. Electronic address:
Photoelectrochemical (PEC) immunosensors are highly promising tools for monitoring biochemical molecules. Constructing high-performance heterojunctions is a general method to improve the sensitivity of PEC immunosensors. The internal electric field (IEF) formed at the heterojunction interface plays a crucial role in coordinating the separation of photogenerated carriers.
View Article and Find Full Text PDFMikrochim Acta
January 2025
College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Zhejiang Normal University, Jinhua, 321004, China.
Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of AgS/FeOOH heterojunction. The AgS/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb.
View Article and Find Full Text PDFMikrochim Acta
November 2024
Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Province, College of Chemistry, Chemical Engineering, and Environment, Minnan Normal University, Zhangzhou, 363000, People's Republic of China.
A photocurrent enhancing photoelectrochemical (PEC) immunosensor was developed for chloramphenicol (CAP) detection based on cation exchange reaction. The efficient split-type PEC immunosensor combined with controlled-release strategy was established using the ZnInS/TiO/TiC MXene (ZIS/T/M) composite as the photoactive material and CuO as the signal response probe. In the presence of target CAP, CuO-labeled CAP antibody (CuO-mAb) was introduced onto the microplate via a competitive-type immunoassay.
View Article and Find Full Text PDFAnal Chim Acta
November 2024
Department of Hepatology, Hepatology Research Institute, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian Province, China; Department of Hepatology, National Regional Medical Center, Binhai Campus of the First Affiliated hospital, Fujian Medical University, Fuzhou 350212, Fujian Province, China; Clinical Research Center for Liver and Intestinal Diseases of Fujian Province, Fuzhou, 350005, Fujian Province, China. Electronic address:
Background: Designing heterojunctions with efficient electron-hole separation holds great promise for improving photoelectric response.
Results: Herein, we reported a multifunctional Pt co-catalyst-modified BiOS nanoflowers (BOS NFs) photocatalytic component for achieving an efficient photoelectric chemistry (PEC) immunosensor for alpha-fetoprotein (AFP). Briefly, the Pt co-catalyst improved the intrinsic band gap structure of BOS on the one hand, and on the other hand, it was able to achieve a rapid decomposition of hydrogen peroxide to hydroxyl radicals, which led to the improvement of electrochemical half-responses during the amplification of target immunosignals.
Biosens Bioelectron
January 2025
Department of Pharmacy, The First Hospital of China Medical University, Shenyang, Liaoning, 110001, PR China; School of Pharmacy, China Medical University, Shenyang, Liaoning, 110122, PR China. Electronic address:
Here, a photoelectrochemical (PEC) immunosensor based on the FJU-200@CdSe heterostructure was developed for epidermal growth factor receptor (EGFR) detection. This is the first application of FJU-200 in PEC. After modification using CdSe quantum dots (QDs), FJU-200 and CdSe QDs formed an S-scheme heterostructure due to the interleaved energy band structure and the difference in Fermi energy (Ef) levels, which generated an efficient and stable PEC signal.
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