Cell membrane biomimetic magnetic fluorescent bifunctional nanoplatform for drug lead discovery.

Backgroud: Biomimetic nanoplatforms based on membrane coating strategies have received increasing attention in the field of medical research. However, it cannot perform biomedical imaging screening, which is essential for real-time identification. As a rich source of new drug discovery, traditional Chinese medicine (TCM) has made important contributions to the treatment of many diseases.

Resullt: Therefore, a magnetic fluorescent bifunctional nanomaterial was developed for screening the active ingredients in Fuzi. The magnetic fluorescence nanoparticles FeO@GO@RhB synthesized by one-step synthesis showed low toxicity, excellent fluorescence properties and fast magnetic separation. After coating the magnetic nanoparticle with cell membrane, the screening procedure was optimized with positive drug verapamil hydrochloride. Under the optimal conditions, the active ingredients in aconite were screened, and the three active ingredients were identified as benzoyl-mesaconine, benzoyl-aconine and benzoyl-hypacoitine by UPLC-MS/MS. In addition, in situ imaging technique was used to further verify the proliferative activity of the screened active ingredients.

Significance: The magnetic fluorescence bifunctional nanoplatform for cell membrane bionic screening combined with in situ imaging technology established in this paper not only can rapidly and effectively target screening and separation of active ingredients in TCM, but also provides a new platform for bioimaging screening.