Comparison between ZenoTOF 7600 system and QTOF for plant metabolome: an example of metabolomics applied to coffee leaves.

Introduction: ZenoTOF is new class of high-resolution mass spectrometer that combines resolution and sensitivity. This mass spectrometer is well designed to perform metabolomics.

Methods: In this context, we compared the performance of ZenoTOF 7600 system (Sciex) with QTOF6520 (Agilent Technologies) through the leaf metabolome analysis of two Coffea species, namely C. anthonyi and C. arabica.

Results: Both species were used to compare both TOF systems. Our results showed that the ZenoTOF 7600 system provided more features (3146 vs 2326 metabolites) and more nodes (1410 vs 379 metabolites) by molecular network in only one injection.

Conclusion: These performances were attributed to the scan speed and sensitivity of the ZenotTOF and demonstrates its added value in the context of metabolomics.