Adsorption Force of Fibronectin Regulates Protein Reorganization, Desorption, and Endocytosis.

Langmuir

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Lab for Smart & Bioinspired Materials, College of Bioengineering, Chongqing University, Chongqing 400030, China.

Published: January 2025

Protein adsorption on biomaterials occurs before cell adhesion. To adapt the properties of biomaterials, adhered cells may utilize and modify adsorbed proteins for survival and function. In this process, the protein-material interfacial force () is supposed to play vital roles, which, however, has received little attention. Here, we found that rat mesenchymal stem cells (rMSCs) can utilize the adsorbed fibronectin (FN) via reorganization, desorption, or endocytosis, and these utilization processes are regulated by through regulating cell adhesion and force balance between the cell traction force and . Furthermore, protein utilization has an -dependent temporal sequence. On low surface, FN endocytosis might happen prior to FN desorption and aggregation. This work confirms the importance of in protein utilization and provides new insight into the mechanism by which cells process their surrounding ECM proteins, which may help to guide the design of better biomaterials.

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http://dx.doi.org/10.1021/acs.langmuir.4c02526DOI Listing

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