Protein adsorption on biomaterials occurs before cell adhesion. To adapt the properties of biomaterials, adhered cells may utilize and modify adsorbed proteins for survival and function. In this process, the protein-material interfacial force () is supposed to play vital roles, which, however, has received little attention. Here, we found that rat mesenchymal stem cells (rMSCs) can utilize the adsorbed fibronectin (FN) via reorganization, desorption, or endocytosis, and these utilization processes are regulated by through regulating cell adhesion and force balance between the cell traction force and . Furthermore, protein utilization has an -dependent temporal sequence. On low surface, FN endocytosis might happen prior to FN desorption and aggregation. This work confirms the importance of in protein utilization and provides new insight into the mechanism by which cells process their surrounding ECM proteins, which may help to guide the design of better biomaterials.
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http://dx.doi.org/10.1021/acs.langmuir.4c02526 | DOI Listing |
Langmuir
January 2025
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Lab for Smart & Bioinspired Materials, College of Bioengineering, Chongqing University, Chongqing 400030, China.
Protein adsorption on biomaterials occurs before cell adhesion. To adapt the properties of biomaterials, adhered cells may utilize and modify adsorbed proteins for survival and function. In this process, the protein-material interfacial force () is supposed to play vital roles, which, however, has received little attention.
View Article and Find Full Text PDFACS Appl Mater Interfaces
December 2024
Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1a, Aachen, 52074, Germany.
As a model for the energy-efficient aftertreatment of exhaust gas components, we studied microwave-assisted (MW) CO oxidation over a (La,Sr)CoO (LSC) perovskite oxide catalyst under dry and humidified conditions. We found that the use of a MW-based process can offer multiple advantages over traditional thermocatalysis in this scenario, as the nature of the MW-solid interaction offers quick, adaptive, and energy-efficient heating as well as improved yield and lower light-off temperatures. As found by combined CO and water MW-desorption experiments, the presence of technically relevant amounts of water leads to a competition for surface active sites and thus slows the reaction rate without indications for a fundamental change in the mechanism.
View Article and Find Full Text PDFEcotoxicol Environ Saf
November 2024
Analytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, Belgium.
In the soil environment, microplastics (MPs) commonly coexist with organic pollutants such as nonylphenol (NP), affecting the migration of NP through adsorption/desorption. However, few studies have focused on the interaction between NP and MPs in soil, especially for MPs of different types and ageing characteristics. In this study, non-polar polypropylene (PP) and polar polyamide (PA) MPs were aged either photochemically (144 h) or within soil (60 days), then used to determine the effect of 5 % MPs on the adsorption behaviour of NP (0.
View Article and Find Full Text PDFPNAS Nexus
April 2024
Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, 35392 Giessen, Germany.
Schistosomiasis, a widespread neglected tropical disease, presents a complex and multifaceted clinical-pathological profile. Using hamsters as final hosts, we dissected molecular events following infection in the liver-the organ most severely affected in schistosomiasis patients. Employing tandem mass tag-based proteomics, we studied alterations in the liver proteins in response to various infection modes and genders.
View Article and Find Full Text PDFNanoscale
March 2024
Institute of Photoelectronic Thin Film Devices and Technology, Solar Energy Conversion Center, Nankai University, Tianjin 300350, P. R. China.
Recently, metal-halide perovskites have rapidly emerged as efficient light emitters with near-unity quantum yield and size-dependent optical and electronic properties, which have attracted considerable attention from researchers. However, the ultrafast nucleation rate of ionic perovskite counterparts severely limits the in-depth exploration of the growth mechanism of colloidal nanocrystals (NCs). Herein, we used an inorganic ligand nitrosonium tetrafluoroborate (NOBF) to trigger a slow post-synthesis transformation process, converting non-luminescent CsPbBr NCs into bright green luminescent CsPbBr NCs to elucidate the concrete transformation mechanism four stages: (i) the dissociation of pristine NCs, (ii) the formation of Pb-Br intermediates, (iii) low-dimensional nanoplatelets (NPLs) and (iv) cubic CsPbBr NCs, corresponding to the blue-to-green emission process.
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