Within the realm of lateral flow assay (LFIA), the conjugation efficiency between signal tracers and antibody constitutes a pivotal determinant for the sensitivity of the detection system. In this study, three-dimensional (3D) complex flower-like MoS self-assembled from 2D MoS, and natural plant polyphenols "Tannic acid" were introduced for surface modification. This composite material exhibits distinct colorimetric signals, excellent monoclonal antibody coupling efficiency, and commendable photothermal properties.Finally, it was used as a signal tracer to establish a 12-min colorimetric/photothermal dual-mode LFIA platform (MoS/TA-LFIA) for Salmonella Typhimurium detection. Compared to the AuNPs-based LFIA, the dual-mode detection platform limits are decreased by 20-fold (to 5 × 10 cfu/mL) and 100-fold (to 10 cfu/mL), respectively. Furthermore, the MoS/TA-LFIA exhibits satisfactory recovery and stability when applied to milk and orange juice samples. Thus, this study provides a novel, efficient and reliable antibody loading strategy for LFIA detection of foodborne pathogens.
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