A comprehensive study of liver-gut microbiota and antioxidant enzyme activity mediated regulation of late-laying hens by high and low residual feed intake.

Residual feed intake (RFI) is a better indicator of feed efficiency than feed conversion ratio (FCR). It is frequently used to evaluate the efficacy of poultry and livestock feed consumption. Generally, Low RFI (LRFI) is associated with better feed conversion efficiency, whereas high RFI (HRFI) suggests poorer feed conversion efficiency. The study examined the association between microorganisms, tissue and organ functions. The results demonstrated that in contrast to the HRFI group, the LRFI group revealed higher length measurements, the digestive organs' mass, and chest width. The antioxidant indices revealed that the enzymatic activities (catalase and glutathione peroxidase) in the LRFI group were significantly higher than those in the HRFI group. The serum levels of HDLC, AST, and ACTH were identified as potential markers that could affect RFI. The variations between high and low RFI and the function of the liver and cecum microbiota of hens during late laying period were systematically investigated by multiple omics techniques. Through 16S, the most common beneficial microbial population in the gut of LRFI groups, such as Oscillospirales, Ruminococcaceae, and Butyricicoccaceae, has been detected via a microbiome-metabolome association analysis. Through multi-omics analysis, we found that FABP1 and ACSS2 are important regulatory genes affecting RFI. These findings will provide a basis for comprehending the role of gut microbiota in regulating RFI and the molecular mechanism behind the phenotypic changes observed in late-laying hens.