Evidence for Bxy-npr-21 on controlling juveniles' growth and modulating male sexual arousal: from molecules to behaviors.

Background: Bursaphelenchus xylophilus is considered a quarantine plant nematode species, that causes major damage to pine ecosystems globally. However, there are few reports on the identification and function of the sex pheromone receptors involved in mating. The function of Bxy-npr-21 as a potential sex pheromone receptor gene was verified from molecules to behaviors in this study.

Results: Here, we firstly report that Bxy-npr-21 is a receptor gene involved in sexual attraction. The bioinformatic analysis indicated that the Bxy-npr-21 gene in B. xylophilus encodes a GPCR. The expression characterization for Bxy-npr-21 showed that it is widely expressed in whole body of larvae and sex organs of adults. The RNAi results suggested that the Bxy-npr-21 gene was involved mainly in movement, feeding, and mating. Sexual arousal experiments further validated that the Bxy-npr-21 gene was involved in the activation of males by female chemical signaling.

Conclusions: Our results strongly suggest that the Bxy-npr-21 gene is a key gene that regulates nematode growth, development and reproduction. The results of this study lay the foundation for revealing the molecular mechanisms of growth and reproduction of B. xylophilus. It can also provide an important basis for further control of B. xylophilus. © 2025 Society of Chemical Industry.