Genome mining of NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A-F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by -based configuration analysis, ROESY NMR, Mosher's ester derivatization, and bioinformatic predictions. Bioinformatic analysis and a N-serine labeling experiment resulted in the identification of a putative -acyltransferase polyketide synthase (-AT PKS) biosynthetic gene cluster, and a biosynthetic pathway is proposed.
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Neptunizhulides, Cryptic -AT Polyketide Synthase-Derived Metabolites from NBU2194.
Org Lett
January 2025
Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center, Ningbo University, Ningbo, Zhejiang 315211, China.
Genome mining of NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A-F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by -based configuration analysis, ROESY NMR, Mosher's ester derivatization, and bioinformatic predictions.
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