Maternal depression has been linked with increased risk of childhood obesity. Furthermore, maternal negative affectivity in early childhood has been associated with food fussiness. We explored the relationship between longitudinal maternal well-being mid-pregnancy, at 2 years and 5 years postpartum and children's appetitive traits at 5 years of age. This is a secondary analysis of the ROLO (randomized control trial of low glycemic diet in pregnancy) longitudinal birth cohort study including pregnancy ( = 279), 2 years ( = 168), and 5 years ( = 295) postpartum. Maternal well-being was measured using the World Health Organization-Five Well-Being Index. Children's appetitive traits were measured at 5 years of age using the Children's Eating Behavior Questionnaire. Bivariate correlation and then linear regression, adjusted for potential confounders, were used to determine relationships between maternal well-being at each timepoint and offspring's appetitive traits at 5 years old. Mean maternal well-being was higher at 5 years postpartum (63.3%) than mid-pregnancy (58.2%) or at 2 years postpartum (61.0%) ( = 0.001). No relationships were observed between maternal well-being mid-pregnancy and child's appetitive traits at 5 years. In adjusted regression, at 2 years postpartum, maternal well-being was negatively associated with "Emotional Overeating" ( = -0.029, = 0.024), "Satiety Responsiveness" ( = -0.040, = 0.014), "Slowness Eating" ( = -0.072, = <0.001), and "Emotional Undereating" ( = -0.054, = 0.010) and positively associated with "Enjoyment of Food" ( = 0.050, = 0.007). Maternal well-being at 5 years postpartum was negatively associated with "Satiety Responsiveness" ( = -0.025, = 0.033) and "Slowness Eating" ( = -0.031, = 0.024). Maternal well-being in early childhood may influence children's appetitive traits; however, further research is required to fully explore this relationship.

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