Analyzing the chemical composition of different kinds of acrylic cement is necessary to understand their properties and suitability for curing bone defects. Conducting various chemical tests can give valuable insight into the composition, viscosity, and performance characteristics of each kind of cement, Therefore, our study aimed to find safety standards and the effectiveness of these products for medical applications. The polymeric characterization was determined by Nuclear Magnetic Resonance (H-NMR) spectroscopy and Fourier-transform infrared spectroscopy (FTIR). Additionally, gel permeation chromatography (GPC) was used to determine the molecular weight of poly methyl methacrylate (PMMA), which was between 4000 and 6000 Mw. The presence of methyl methacrylate (MMA) monomer observed in all cement within two minutes was determined using gas chromatography-mass spectrometry (GC-MS). Moreover, the images of all radiopaque compounds in the cement were evaluated using Field emission scanning electron microscopy (FESEM) and Energy Dispersive X-ray (EDAX-MAP). The study determined the glass transition (Tg) temperature and conducted differential scanning calorimetry (DCS) analysis for each type of cement. In addition, the setting time for various kinds of spinal cord cement was measured to be more than ten minutes. The percentage of benzoyl peroxide in each cement was determined using titration, ranging from 0.6% to 6%. Additionally, cytotoxicity studies were conducted on human osteoblasts (MG63) in cell culture. In this study, we tried to make a trend line for evaluation types of bone cement that would be applicable for both regulatory buddies and researchers in this field.
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