Int J Biol Macromol
Institute of Botany, Jiangsu Province, Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China; Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-cultivation and High-value Utilization of Chinese Medicinal Materials, Nanjing 210014, China. Electronic address:
Published: January 2025
WRKY transcription factors (TFs) play pivotal roles in regulating plant nutrient uptake, particularly phosphate (Pi) acquisition, and biosynthesis of secondary metabolites. Euphorbia lathyris, a significant medicinal plant with diverse pharmacological activities, lacks a systematic analysis of WRKY members and their functional roles. In this study, 58 ElWRKY genes were identified in the E. lathyris genome, classified into seven subgroups through comparative genomics analysis, and distributed on 10 chromosomes. Phylogenetic and expression pattern analyses identified ElWRKY48 as a candidate gene involved in Pi uptake regulation. The transgenic validation assay demonstrated that ElWRKY48 overexpression negatively regulated Pi uptake and led to phosphorus-deficient phenotypes in the hairy roots of E. lathyris. Furthermore, the transcriptome analysis revealed an opposite expression pattern between the Pi transporter gene ElPHT1 (Elat0034050.1) and ElWRKY48 in the transgenic lines overexpressing ElWRKY48. The negative regulation of ElPHT1 expression by ElWRKY48 was validated through qRT-PCR, Y1H, EMSA, gene knockout (CRISPR/Cas9), and LUC assays. Additionally, the overexpression of ElWRKY48 reduced diterpenoid ingenol biosynthesis by suppressing the expression of its biosynthesis-related genes. These findings provide valuable insights into the role of WRKY in Pi uptake and offer potential avenues for genetic improvement in the yield and quality of E. lathyris.
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http://dx.doi.org/10.1016/j.ijbiomac.2025.139859 | DOI Listing |
Sci Rep
January 2025
Agricultural College of Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
Salinity tolerance in brewing sorghum is a very important trait, especially in areas that are affected by soil salinity. In order to elucidate the mechanism underlying salt tolerance, we conducted a comparative analysis of the transcriptome and metabolome in two distinct sweet sorghum genotypes, namely the salt-tolerant line NY1298 and the salt-sensitive line MY1176, following exposure to salt treatment. Our initial findings indicate the presence of genotype-specific responses in brewing sorghum under salt stress conditions.
View Article and Find Full Text PDFPlant Genome
March 2025
Department of Soil, Plant and Food Sciences, Genetics and Plant Breeding Section, University of Bari Aldo Moro, Bari, Italy.
Wheat breeders are constantly looking for genes and alleles that increase grain yield. One key strategy is finding new genetic resources in the wild and domesticated gene pools of related species with genes affecting grain size. This study explored a natural population of Triticum turgidum (L.
View Article and Find Full Text PDFPlants (Basel)
January 2025
Corteva Agriscience, 7000 NW 62nd Ave, Johnston, IA 50131, USA.
Maize lethal necrosis (MLN) is a significant threat to food security in Sub-Saharan Africa (SSA), with limited commercial inbred lines displaying tolerance. This study analyzed the transcriptomes of four commercially used maize inbred lines and a non-adapted inbred line, all with varying response levels to MLN. RNA-Seq revealed differentially expressed genes in response to infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV), the causative agents of MLN.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Salt stress is a significant environmental factor that impedes maize growth and yield. Exogenous 5-aminolevulinic acid (ALA) has been shown to mitigate the detrimental effects of various environmental stresses on plants. However, its regulatory role in the photosynthesis mechanisms of maize seedlings under salt stress remains poorly understood.
View Article and Find Full Text PDFBiotechnol Adv
January 2025
State Key Laboratory of Crop Gene Resources and Breeding/Key laboratory for Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, PR China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:
Sustainable agriculture practices are indispensable for achieving a hunger-free world, especially as the global population continues to expand. Biotic stresses, such as pathogens, insects, and pests, severely threaten global food security and crop productivity. Traditional chemical pesticides, while effective, can lead to environmental degradation and increase pest resistance over time.
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