Background: Circulating tumor DNA (ctDNA) is a promising biomarker for cancer prognosis and drug development. A major challenge in the ctDNA determination method is discriminating ctDNA from highly similar but significantly more abundant wild-type DNA sensitively and accurately.
Method: An ultrasensitive qPCR method termed Triple Enrichment Amplification of Mutation PCR (TEAM-PCR) was developed to detect EGFR T790M mutation.
Results: EGFR T790M was quantified over the assay range of 25-10 copies/reaction in the presence of 10 wild-type copies. This method was fully validated following the essential bioanalysis guidance, with the limit of detection (LOD) being five copies/reaction.
Conclusion: This study established and validated a qPCR-based strategy to detect EGFR T790M mutation with ultra-high sensitivity and reliability.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749345 | PMC |
| http://dx.doi.org/10.1080/17576180.2025.2451527 | DOI Listing |
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